The effect of dynamic lockdowns on public transport demand in times of COVID-19: Evidence from smartcard data.

Governments around the globe have taken different measures to tackle the COVID-19 pandemic, including the lockdown of people to decrease infections. The effect of such a strategy on transport demand is important not only for the current pandemic but also to understand changes in transport use and for future emergencies. We analyse a 2019-2020 database of smartcard data of trips from the city of Santiago, Chile, which followed a dynamic lockdown strategy in which its municipalities were temporarily restricted.

Enhancement of connexin 43 expression increases proliferation and differentiation of an osteoblast-like cell line.

Bone cells form a functional syncytium as they are coupled by gap junctions composed mainly of connexin 43 (Cx43). To further understand the role of Cx43 in bone cell growth and differentiation, we stably transfected Cx45-expressing UMR 106-01 cells with Cx43 using an expression vector containing rat Cx43 cDNA. Three stably transfected clones were analyzed, all of which showed altered expression of Cx43 and/or Cx45 as was obvious from immunocytochemistry and Northern blotting.