Publications by authors named "B Gellersen"
Study Question: Do maternal endometrial epithelial cell (EEC) differentiation and polarity impact the invasive capacity of extravillous trophoblast (EVT) cells during early human implantation?
Summary Answer: In a three dimensional (3D) confrontation co-culture the invasiveness of the human trophoblast cell line AC-1M88 was inversely correlated with the degree of differentiation and polarization of human endometrial adenocarcinoma cell spheroids.
What Is Known Already: In a previous study desmosomal and adherens junction proteins were shown to spread from a subapically restricted lateral position to the entire lateral membrane in human glandular EECs during the implantation window of the menstrual cycle. Whether this change in EEC junction localization has an impact on the interaction of EVT cells with glandular EECs during early human implantation is not known.
Decidualization denotes the transformation of endometrial stromal fibroblasts into specialized secretory decidual cells that provide a nutritive and immunoprivileged matrix essential for embryo implantation and placental development. In contrast to most mammals, decidualization of the human endometrium does not require embryo implantation. Instead, this process is driven by the postovulatory rise in progesterone levels and increasing local cAMP production.
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- Mechanisms behind early reproductive loss are being unraveled, with a focus on the role of endometrial stromal cells (ESCs) in tissue remodeling related to embryo implantation and regeneration.
- Studies from 2000-2012 indicate that abnormal ESC migration and invasion contribute to issues like implantation failure and recurrent pregnancy loss (RPL), as well as conditions like endometriosis and adenomyosis.
- Understanding the complex signaling pathways controlling ESC behavior may lead to new treatments for reproductive disorders, highlighting the importance of targeted regulatory molecules.
Article Synopsis
- The study investigates how endometrial stromal cells, which contribute to the implantation process, exhibit motility and invasiveness and are influenced by trophoblast-derived factors.
- Key growth factors such as HB-EGF and PDGF-BB are identified, with HB-EGF causing directed movement (chemotaxis) and PDGF-BB inducing both directed and non-directed movement (chemokinesis) in these cells.
- Proteome profiling reveals other important factors like placental growth factor and PDGF-AA from trophoblasts, with PDGF-AA specifically promoting chemotaxis but indicating that there are likely more unknown trophoblast factors involved in this process.
We have previously shown that the presence of trophoblast cells enhances invasiveness of decidualizing human endometrial stromal cells. The metastasis suppressor CD82, which has antimigratory function in tumor cells, is up-regulated in decidualizing endometrial stromal cells. CEACAM1 is expressed in trophoblast cells at the invasion front in early placenta and is considered proinvasive.
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