Publications by authors named "B G Dekker"
Article Synopsis
- Detailed method descriptions help scientists repeat experiments and get similar results.
- The European Commission PRO-MaP report gives important tips for researchers in life sciences.
- These tips help improve research quality and make it easier to share and reuse data.
The topological state of chromosomes determines their mechanical properties, dynamics, and function. Recent work indicated that interphase chromosomes are largely free of entanglements. Here, we use Hi-C, polymer simulations, and multi-contact 3C and find that, by contrast, mitotic chromosomes are self-entangled.
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- Mitotic chromosomes in early embryogenesis scale down in size alongside the cell, spindle, and nucleus, but have unique mechanisms that differ from other subcellular structures.
- Research shows that while the size of mitotic chromosomes adjusts with the nuclear-cytoplasmic ratio, it cannot be reset by earlier developmental stages like spindles and nuclei.
- The study highlights that mitotic chromosomes shrink due to reduced recruitment of condensin I, leading to changes in DNA structure to fit into the smaller chromosome axes as development progresses.
Over the last several years enormous progress has been made in identifying the molecular machines, including condensins and topoisomerases that fold mitotic chromosomes. The discovery that condensins generate chromatin loops through loop extrusion has revolutionized, and energized, the field of chromosome folding. To understand how these machines fold chromosomes with the appropriate dimensions, while disentangling sister chromatids, it needs to be determined how they are regulated and deployed.
View Article and Find Full Text PDFObjective: Thyroglobulin (Tg) is an established tumor marker for differentiated thyroid carcinoma (DTC) patients. However, Tg immunoassays can be subject to Tg autoantibody (TgAb) interference resulting in incorrect Tg values. Therefore, Tg measurement with liquid chromatography-tandem mass spectrometry (LC-MS/MS) could be promising in patients with TgAbs.
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