Publications by authors named "B G Blanchy"

Factor XIII from placenta was successfully grafted onto collagen membranes by the acyl-azide procedure. The transamidase activity retained on collagen membranes was determined by measuring the increase of fluorescence resulting from dansylcadaverine incorporation into casein. We studied the effect of different factors on the grafting: concentration and composition of the factor XIII preparation in the coupling solution and influence of the preactivation of factor XIII by thrombin.

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Two fractions, I and II, corresponding to heavy and light molecular forms of HBeAg and respectively associated with the (HBe/1 + HBe/2) and (HBe/3) specificities, were separated by ammonium sulfate precipitation in HBeAg positive chimpanzee plasma. I 125-labeling of fractions I and II and immune precipitation by anti-HBe and anti-Human IgG were followed by autoradiographic analysis after SDS polyacrylamide gel electrophoresis: This revealed that two small peptides (a major 17,000 MW and minor 21,000 MW) seem common to the various molecular forms and specificities of HBeAg. Both peptides were found tightly linked to IgG in fraction I, associated with HBe/1 and HBe/2 and precipitated by 1.

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