Velocity-informed upper bounds on the convective heat transport induced by internal heat sources and sinks.

Three-dimensional convection driven by internal heat sources and sinks (CISS) leads to experimental and numerical scaling laws compatible with a mixing-length-or 'ultimate'-scaling regime [Formula: see text]. However, asymptotic analytic solutions and idealized two-dimensional simulations have shown that laminar flow solutions can transport heat even more efficiently, with [Formula: see text]. The turbulent nature of the flow thus has a profound impact on its transport properties.

Disruption of the mycobacterial cell entry gene of Mycobacterium bovis BCG results in a mutant that exhibits a reduced invasiveness for epithelial cells.

Mycobacteria belonging to the Mycobacterium tuberculosis complex have the ability to invade and replicate in non-phagocytic cells, an event that requires the presence of bacterial surface components capable of triggering a cell response and the subsequent internalization of the microorganism. In this study, we report the sequencing of the mycobacterial cell entry gene (mce) of Mycobacterium bovis bacillus Calmette-Guérin (BCG) and the generation and characterization of a mutant BCG strain with an inactivated mce gene, by homologous recombination with double cross-over. This mutant strain does not express the mycobacterial cell entry protein (Mce) and exhibits a reduced ability to invade the non-phagocytic epithelial cell line HeLa as compared to wild-type BCG.