Oral 2-chlorodeoxyadenosine in psoriatic arthritis. A preliminary report.

Objective: To determine if weekly oral 2-chlorodeoxyadenosine (2-CdA) can induce selective lymphocytopenia, and reduce inflammation, in patients with refractory psoriatic arthritis.

Methods: Seven patients with psoriatic arthritis were treated with oral 2-CdA at weekly dosages of 0.3 mg/kg to 0.

Characterization of calmodulin-dependent and cyclic-AMP-dependent protein kinase stimulation of cardiac sarcoplasmic reticulum calcium transport.

The mechanism by which calmodulin stimulates Ca2+ transport in cardiac microsomal preparations enriched in sarcoplasmic reticulum (SR) was investigated. Under incubation conditions in which the majority of the phosphoprotein formed was Ca2+-dependent and no phospholamban phosphorylation was observed (10 degrees C, 15-sec incubations in the presence of 2 microM ATP), calmodulin was found to have no effect on the steady-state level of the acylphosphate phosphorylation site of Ca2+-ATPase. A significant stimulation of Mg2+, Ca2+-ATPase activity by calmodulin and a 3-fold increase in the turnover of the Ca2+ pump were, however, observed.

Ca2+-transport in skeletal muscle sarcoplasmic reticulum of the chronically diabetic rat.

Ca2+-transport in fast skeletal muscle sarcoplasmic reticulum preparations was studied in experimentally-induced diabetic rats 90 days after treatment. A significant decrease in ATP-dependent oxalate facilitated Ca2+-uptake was observed in these animals at all free Ca2+ concentrations tested (0.1-2.

The presence and binding characteristics of calmodulin in microsomal preparations enriched in sarcoplasmic reticulum from rabbit skeletal muscle.

ATP-dependent oxalate facilitated calcium transport in sarcoplasmic reticulum (SR) preparations obtained from rabbit vastus lateralis muscle (fast skeletal muscle; Fsr) and soleus (slow skeletal muscle; Ssr) was determined. Addition of exogenous calmodulin did not stimulate calcium transport in either Fsr or Ssr preparations. Fsr and Ssr previously washed in 1 mM EGTA demonstrated a reduced capacity to transport Ca2+; the exogenous addition of calmodulin (0.

Depression of calcium transport in sarcoplasmic reticulum from diabetic rats: lack of involvement by specific regulatory mediators.

Cardiac sarcoplasmic reticulum (SR) ATP-dependent Ca2+-uptake was found to be depressed in 4 month streptozotocin-induced diabetic rats. Calmodulin, cAMP-dependent protein kinase and K+ stimulated Ca2+-uptake to similar degrees in SR from both control diabetic rats. Long chain acylcarnitine (7 microM) decreased Ca2+-transport in control rats by 46% but only 26% in diabetic animals.