Development of simplex and quintuplex RT-PCR for simultaneous detection of soybean viruses.

Five simplex and a multiplex-RT-PCR (m-RT-PCR) protocols were developed for detection and differentiation of bean pod mottle virus (BPMV), cherry leaf roll virus (CLRV), raspberry ringspot virus (RpRSV), soybean mosaic virus (SMV) and tomato ringspot virus (ToRSV) infecting soybean. The simplex RT-PCR protocols produced virus-specific amplicons of 538 bp for BPMV, 139 bp for CLRV, 298 bp for RpRSV, 403 bp for SMV, and 282 bp for ToRSV, with sensitivity down to 10 diluted cDNA. Further, to detect all the five viruses simultaneously in a single tube a quintuplex RT-PCR protocol was optimized with as low as 10 diluted cDNA and 0.

Advances in plant pathogen detection: integrating recombinase polymerase amplification with CRISPR/Cas systems.

Plant pathogens are causing substantial economic losses and thus became a significant threat to global agriculture. Effective and timely detection methods are prerequisite for combating the damages caused by the plant pathogens. In the realm of plant pathogen detection, the isothermal amplification techniques, e.

Green Synthesis of Ganoderma Lucidum-Mediated Silver Nanoparticles and its Microbial Activity against Oral Pathogenic Microbes: An Study.

Nanotechnology is developing into a fast-expanding discipline with applications in science and technology, and nanostructures are a crucial research tool in many fields. Due to their remarkable electrical, optical, magnetic, catalytic, and pharmacological capabilities, metal and metal oxide nanoparticles (NPs) have drawn study interest. Natural elements (plants, microorganisms, fungi, etc.

Antimicrobial properties of green synthesized silver and chitosan nanocomposites.

An eco-friendly and simple approach was carried out for the synthesis of silver-chitosan nanocomposites using and fluconazole-mediated aqueous extract. This extract acted as a reducing agent as well as a capping agent for the green synthesis of silver nanoparticles. Chitosan nanoparticles on the other hand were synthesized from the deacetylation of the chitin matrix.