Relationships between Brucella ovis semen culture and various semen and serology parameters.

Semen and blood samples from 154 rams from two Montana range flocks (Flock A, vaccinated for Brucella ovis ; Flock B, nonvaccinated) were evaluated to determine the relationship between Brucella ovis (B. ovis ) semen culture results and various semen and blood parameters. All rams utilized in this study exhibited no palpable ram epididymitis lesions.

Vaccination of pregnant guinea pigs with Campylobacter fetus: effects of antigen dose, Campylobacter strain, and adjuvant type.

For the purpose of developing and improving vaccines to protect cattle against bovine genital campylobacteriosis, the interaction of vaccine dose, strain(s) of Campylobacter fetus, and type of adjuvant was studied in the pregnant guinea pig model. Five strains of C fetus were used in the preparation of 20 monovalent vaccines containing various doses of bacteria (0.1 mg to 20 mg, dry weight) in Freund incomplete adjuvant, Freund complete adjuvant, or aluminum hydroxide gel adjuvant.

Inhibition of growth and swarming of Proteus mirabilis and Proteus vulgaris by triclosan.

The MICs of triclosan (2,4,4'-trichloro-2'-hydroxydiphenyl ether) and the effect on swarming were determined for 35 isolates of Proteus mirabilis and 7 isolates of P. vulgaris of animal origin. Both species were susceptible to the antimicrobial agent, and growth of all but one isolate was inhibited by less than 1 microgram/ml in broth and on agar without blood.

Bulk growth procedures and a button agglutination test for Campylobacter.

Bulk-cell yields were obtained from 4 Campylobacter spp incubated aerobically without the use of a special atmosphere. A button agglutination test was developed for quantitation of blood serum antibodies against C fetus subsp venerealis, C fetus subsp fetus, C jejuni, and "C hyointestinalis." The test was sensitive, and different individuals reading it usually attained the same titers.