Publications by authors named "B Crego-Vicente"
Background: Malaria is the parasitic disease with the highest morbimortality worldwide. The World Health Organization (WHO) estimates that there were approximately 249 million cases in 2022, of which 3.4% were in Angola.
View Article and Find Full Text PDFBackground: Species hybridization represents a real concern in terms of parasite transmission, epidemiology and morbidity of schistosomiasis. It is greatly important to better understand the impact of species hybridization for the clinical management.
Methods: A prospective observational study was carried out in sub-Saharan migrants who were diagnosed with confirmed genitourinary schistosomiasis.
The loop-mediated isothermal amplification (LAMP) technique is a great alternative to PCR-based methods, as it is fast, easy to use and works with high sensitivity and specificity without the need for expensive instruments. However, one of the limitations of LAMP is difficulty in achieving the simultaneous detection of several targets in a single tube, as the methodologies that allow this rely on fluorogenic probes containing specific target sequences, complicating their adaptation and the optimization of assays. Here, we summarize different methods for the development of multiplex LAMP assays based on sequence-specific detection, illustrated with a schematic representation of the technique, and evaluate their practical application based on the real-time detection and quantification of results, the possibility to visualize the results at a glance, the prior stabilization of reaction components, promoting the point-of-care use, the maximum number of specific targets amplified, and the validation of the technique in clinical samples.
View Article and Find Full Text PDFArticle Synopsis
- Migratory flows are leading to increased cases of schistosomiasis in non-endemic countries, prompting a study to assess the effectiveness of the LAMP technique for diagnosing this disease.
- The study evaluated urine samples from sub-Saharan migrants in Spain, comparing LAMP to a commercial immunochromatographic test and microscopic examination of stool and urine.
- Results showed that while the LAMP technique had better sensitivity than microscopy for diagnosing urinary schistosomiasis, the immunochromatographic test was more effective overall, suggesting LAMP could improve diagnostics in resource-limited settings.
Article Synopsis
- Strongyloides stercoralis is a widespread but often overlooked tropical disease that can cause mild to severe health issues, especially in immunocompromised individuals.
- The study evaluated a new and simpler diagnostic method called Strong-LAMP for detecting strongyloidiasis in stool samples from Angola, comparing its effectiveness with traditional laboratory methods in Spain.
- A total of 192 stool samples were analyzed, with varying detection rates using different methods; Strong-LAMP showed potential for use in low-resource settings, but results indicated a need for further validation.