Expression of calretinin in human mesothelioma cell lines and cell cycle analysis by flow cytometry.

Human mesothelioma cell lines were studied concerning the expression of the calcium-binding protein calretinin (CR), and the relation of the DNA index to their cell cycle. The results obtained for cell lines with different morphological characteristics, were compared to those from human mesothelial cells transfected with SV40 to escape senescence. Immunocytochemical expression of calretinin (confirmed by immunoblot) was observed in all mesothelioma cell lines but not in the control cells.

Comparative studies of calretinin expression by WiDr cell line in vivo in xenografts in nude mice and in vitro.

WiDr cells from a human colon adenocarcinoma cultivated in vitro express the calcium binding protein calretinin. The immunoreactivity is present in some interphasic cells and decreases after seven days in culture together with the augmentation of the cell number. Calretinin expression is maintained in the undifferentiated cells of the tumoral mass developed in nude mice and in recultivated isolated tumour cells from the xenograft.

Morphological aspects of lymphocyte-macrophage interactions in human tumoral effusions "in vivo" and "in vitro".

"In vivo" and "in vitro" morphological analysis of associations of cells ("rosettes") involved in immune response in human tumoral effusions revealed the existence of cell interactions either by simple membrane apposition between the cell projections or by gap-like junctions between two adjacent cells; endocytotic phenomena were also observed. The giant fibroblastic cells seen "in vitro" ("myofibronoblasts") reacting positively to anti-human macrophage Mabs, might be the cells presenting antigen to lymphocytes.

Microcinematographic and electron microscopic study of the death of human tumor cells induced by histiocytic cells.

The cells from a malignant fibrous histiocytoma were enzymatically isolated and cultured in vitro. The cultures were observed by microcinematography and with an electron microscope after fixation and embedding. The interactions between histiocytic and tumor cells resulted in tumor cell death.