Quantification of beef myofibrillar proteins by SDS-PAGE.

A semi-quantitative determination of beef myofibrillar proteins using sodium, dodecyl sulphate polyacrylamide gel electrophoresis is described. Bovine serum albumin was used as internal standard. Results indicate a linear relationship between densitometric readings after staining with Coomassie brilliant blue R(250) and protein content.

Effect of a β-agonist on meat quality and myofibrillar protein fragmentation in bulls.

Three experiments were conducted to study the effect of cimaterol on meat quality and myofibrillar protein fragmentation of the Longissimus dorsi muscle. In two experiments (Experiments 1 and 2), conducted with 16 double-muscled Belgian white-blue bulls and 15 Charolais bulls, respectively, half of the animals received 60 μg cimaterol daily per kg liveweight in the diet, during 135 and 93 days, respectively. In a third experiment, 46 normal Belgian white-blue bulls received no or 4 ppm cimaterol in the diet for 246, 127 or 71 days on average.

Effects of electrical stimulation, followed by moderate cooling, on meat quality characteristics of veal Longissimus dorsi.

Three groups of male Friesian veal calves (in total, 92) were slaughtered in separate series. Within each group, a subgroup (selected on the basis of blood characteristics determined 1 week ante mortem) was electrically stimulated immediately after bleeding (64 s-Mitab® Low voltage unit). Carcasses were transferred ca.