Liquid chromatographic determination of cyclosporin A in serum with use of a solid-phase extraction. Comparison between high-performance liquid chromatography and radioimmunoassay levels in clinical investigations.

A simple reliable liquid chromatographic method for assay of cyclosporin A in serum or urine is described. Samples were cleaned up on a solid-phase extraction system (cyanopropyl column). The system involved a reversed-phase C18 Ultrasphere column maintained at 72 degrees C and an acetonitrile linear gradient (65 to 95%) in 0.

Determination of free and total polyamines in human serum and urine by ion-pairing high-performance liquid chromatography using a radial compression module. Application to blood polyamine determination in cancer patients treated or not treated with an ornithine decarboxylase inhibitor.

A sensitive and rapid determination of free and total polyamines (putrescine, cadaverine, spermidine and spermine) in urine and serum is described. The procedure is based on reversed-phase high-performance liquid chromatographic separation using radial compression module (Radialpak C 8). The samples are purified with a silica gel Sep-Pak cartridge.