[Experimental research on the toxicity of pharmapentoxifylline (II)].

The results from morphological studies on liver, lung, heart, spleen, intestine, brain, skin, testes (ovaries), obtained in the end of the first and third month of animals, treated with pharmapentoxiphyline (PP), are described. The experiments were carried out on 312 white rats (male and female), divided into 2 series: I series--oral usage of the preparation in doses of 100, 300 and 6 mg/kg, II series--intraperitoneal administration in doses of 30, 75 and 150 mg/kg. It was established that PP, administered for a period of 3 months orally in a dose of 100 mg/kg and intraperitoneally in a dose of 30 mg/kg did not induced changes in the structure of all examined organs.

[Effects of preliminary fasting on the development of D-galactosamine-induced acute lesion of the liver in rats].

The effect of 48-hour preliminary fasting on the development of acute hepatitis in 24 h after the use of 1,855 mmol D-galactosamine per 1 kg bw has been studied on rats. Both intensification of dystrophic and necrotic processes in the liver and aggravation of ultrastructural changes and disorders have been observed. Concentrations of reduced glutathione in the liver and D-glucaric acid in the urine are not changed under the effect of intoxication.

[Effect of prior starvation on the development of acute thioacetamide-induced liver damage in rats].

The effect of previous fasting on the liver morphological changes and microsomal cytochrome P-450 and b5 content was studied in thioacetamide-induced (100 mg/kg) rat liver necrosis. Starvation for 48 hours immediately before thioacetamide administration aggravates the dystrophic and necrotic processes, as revealed by histology, electron microscopic investigations and serum aminotransferase activity. The liver microsomal cytochrome P-450 concentration tended to decrease after thioacetamide challenge, with fasting resulting in a more significant loss of cytochrome P-450.

Immunological and radioimmunological studies in food allergy.

Experiments in order to induce food allergy were carried out in guinea pigs. The sensitization with egg albumin, pasteurized cow milk and bovine serum albumin provoked anaphylactic shock. The passive cutaneous anaphylaxis, serum antibodies, liver cytochrome P-450 concentration and the anaphylactic shock were determined.