[Interface of the interaction of the middle domain of human translation termination factor eRF1 with eukaryotic ribosomes].

Termination of translation in eukaryotes is governed by two polypeptide chain release factors. The middle (M) domain of the class 1 translation termination factor eRF1 contains the strictly conserved GGQ motif and involved in hydrolysis of the peptidyl-tRNA ester bond within the peptidyl transferase center of the large ribosome subunit. Heteronuclear NMR spectroscopy was used to map the interaction interface of the M-domain of human termination factor eRF1 with eukaryotic ribosomes.