Assessing mitochondrial respiration in isolated hearts using (17)O MRS.

The application of (17)O MRI and MRS for the evaluation of cardiac mitochondrial function has been limited because of the challenge of detecting metabolic H(2)(17)O in the vast background of naturally abundant H(2)(17)O. In this study, we have developed a direct (17)O MRS approach to examine the feasibility and sensitivity of detecting metabolically produced H(2)(17)O in isolated rat hearts perfused with (17)O(2)-enriched Krebs-Henseleit buffer containing normal (1.5 mm) and high (2.

Early monitoring of acute tubular necrosis in the rat kidney by 23Na-MRI.

Reabsorption of water and other molecules is dependent on the corticomedullary sodium concentration gradient in the kidney. During the early course of acute tubular necrosis (ATN), this gradient is altered. Therefore, 23Na magnetic resonance imaging (MRI) was used to study the alterations in renal sodium distribution in the rat kidney during ischemia and reperfusion (IR) injury, which induces ATN.

[Noninvasive monitoring of the hepatocellular carcinoma growth by the method of 1H and 23Na nuclear magnetic resonance].

Using diffusion weighted 1H, single-quantum 23Na and triple-quantum-filtered 23Na magnetic resonance imaging water apparent diffusion coefficient (ADC), total tissue Na+ and intracellular Na+ were monitored in hepatocellular carcinoma (HCC) in rats and in the surrounding liver tissue. The tumor water ADC was approximately 50% higher compared to the nearby healthy liver tissue but did not increase during 28 days of tumor growth (double time 3.9 days).