Galloyl Dialkyl Lipids Drive Encapsulation of Peptides into Lipid Nanoparticles by Hydrogen Bonding.

The intracellular delivery of peptides and proteins is crucial for various biomedical applications. Lipid nanoparticles (LNPs) have emerged as a promising strategy for delivering peptides to phagocytic cells. However, the diverse physicochemical properties of peptides necessitate tailored formulations.

Poly(2-Hydroxymethyl-2-Oxazoline) as Super-Hydrophilic Antifouling Polymer.

Non-ionic "super-hydrophilic" polymers generally possess strong non-fouling characteristics and, therefore, can suppress non-specific and unwanted interactions with blood proteins when attached to in vivo nanomedicine ranging from drug or gene delivery to diagnostics. In this contribution, we revitalize a protected alcohol functionalized 2-oxazoline monomer, 2-acetoxymethyl-2-oxazoline, that was first reported almost fifty-five years ago and explore the possibility of making "super-hydrophilic" poly(2-oxazoline)s for biomedical applications. The synthesis of the 2-acetoxymethyl-2-oxazoline monomer and its cationic ring-opening homopolymerization and copolymerization kinetics are reported.

Home phototherapy for neonatal hyperbilirubinemia: current practices and attitudes.

Background: Neonatal hyperbilirubinemia is a leading cause of hospitalization during the first week of life. Recent research suggest that phototherapy, the standard treatment, can be safely and effectively administered at home. Some Dutch hospitals have already adopted home-based phototherapy.

Lysosomal Trafficking and Degradation of Extracellular Proteins via Multivalent Small Molecule Ligand Display on Dextran Scaffolds.

Targeted protein degradation (TPD) marks a shift in drug development from conventional inhibition to the complete removal of pathological proteins. Traditional TPD technologies target intracellular proteins of interest (POIs) for degradation but are ineffective against extracellular cell surface and soluble proteins, a significant portion of the human proteome. Recent advances involve the formation of ternary complexes between a POI and a cell surface lysosomal trafficking receptor, directing POIs to lysosomes for degradation.

Intratumoral delivery of lipid nanoparticle-formulated mRNA encoding IL-21, IL-7, and 4-1BBL induces systemic anti-tumor immunity.

Local delivery of mRNA-based immunotherapy offers a promising avenue as it enables the production of specific immunomodulatory proteins that can stimulate the immune system to recognize and eliminate cancer cells while limiting systemic exposure and toxicities. Here, we develop and employ lipid-based nanoparticles (LNPs) to intratumorally deliver an mRNA mixture encoding the cytokines interleukin (IL)-21 and IL-7 and the immunostimulatory molecule 4-1BB ligand (Triplet LNP). IL-21 synergy with IL-7 and 4-1BBL leads to a profound increase in the frequency of tumor-infiltrating CD8 T cells and their capacity to produce granzyme B and IFN-γ, leading to tumor eradication and the development of long-term immunological memory.