Identification of sensory neurons supplying receptors in lingual muscles of the rat: histochemical and retrograde labeling study with horseradish peroxidase.

Sensory innervation of lingual musculature was studied in young adult Wistar rats using retrograde labeling by horseradish peroxidase (HRP) and combined silver impregnation and acetylcholinesterase (AchE) methods. Intra-lingual injection of HRP resulted in labeling of neuronal somata in the trigeminal, superior vagal, and second cervical spinal (C2) ganglia. When HRP was directly applied to the proximal stump of severed hypoglossal nerve, labeling occurred only in the cervical and superior vagal ganglia.

Ultrastructural localization of adenosine triphosphatase activity in HeLa cells at various stages of the cell cycle.

HeLa cells in a monolayer culture were synchronized to S, G2 and mitotic phases by use of excess (2.5 mM) deoxythymidine double-block technique. The localizations of Ca2++-activated adenosine triphosphatase (ATPase) at different phases of the cell cycle were studied using light- and electron-microscopic histochemical techniques, and microphotometric comparisons of the densities of reaction products.

Histochemical localization of adenosine triphosphatase activity in thymus: a light microscopical and ultrastructural study.

Ultrastructural localization of ATPase at high pH in the presence of Ca2+ showed that activity in thymocyte precursors was stronger than in mature thymocytes. The activity was localized in the nuclear envelope, rough endoplasmic reticulum, Golgi apparatus and mitochondria. The difference in activity was attributed to a marked decrease in ATPase-containing organelles, mainly the endoplasmic reticulum in the mature thymocytes.

The fate of phytohaemagglutinin-activated human lymphocytes following their peak proliferative activity.

Morphological and histochemical studies and cell counts were performed on phytohaemagglutinin-stimulated lymphocyte cultures at intervals from 1 to 17 days. Following a phase of proliferative activity lasting from the second to the sixth days, different cell types became evident in the later stages of culture. Some of the cells were large and characterised by an abundant cytoplasm containing numerous electron-dense bodies with a characteristic internal structure, a well developed Golgi apparatus, a highly irregular plasma membrane, numerous pinocytoses and intense acid phosphatase activity localised within the dense bodies.

Ultrastructural localization of adenosine triphosphatase activity in lymphocytes activated in vitro by phytohaemagglutinin.

The ultrastructural localization of Ca2+, Mg2+-activated ATPase was studied in phytohaemagglutinin activated lymphocytes and in normal unstimulated lymphocytes. Cells, fixed in paraformaldehyde--glutaraldehyde, were incubated in a medium containing 3 mM ATP, 5 mM CaCl2 and 2.4 mM Pb(NO3)2 in 0.