Publications by authors named "B A Karpinski"
Mechanisms Regulating Mitochondrial Transfer in Human Corneal Epithelial Cells.
Invest Ophthalmol Vis Sci
November 2024
Purpose: The intraepithelial corneal nerves (ICNs) innervating the cornea are essential to corneal epithelial cell homeostasis. Rho-associated kinase (ROCK) inhibitors (RIs) have been reported to play roles in neuron survival after injury and in mitochondrial transfer between corneal epithelial cells. In this study, the mechanisms human corneal limbal epithelial (HCLE) cells use to control intercellular mitochondrial transfer are assessed.
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- The study investigates the role of rho kinase (ROCK) inhibitors in promoting the health of corneal nerves and their potential effect on nerve regeneration after injury.
- Researchers analyzed integrin and extracellular matrix expression in mouse neurons and explored how RI treatment influences neurite growth both in lab cultures and in live mice following injuries.
- Results showed that specific integrins were present on neuron growth structures, and RI treatment enhanced nerve growth in vitro and improved sensory nerve recovery in vivo after injury.
In this paper, we use RNAseq to identify senescence and phagocytosis as key factors to understanding how mitomyin C (MMC) stimulates regenerative wound repair. We use conditioned media (CM) from untreated (CMC) and MMC treated (CMM) human and mouse corneal epithelial cells to show that corneal epithelial cells indirectly exposed to MMC secrete elevated levels of immunomodulatory proteins including IL-1α and TGFβ1 compared to cells exposed to CMC. These factors increase epithelial and macrophage phagocytosis and promote ECM turnover.
View Article and Find Full Text PDF22q11.2 Deletion Syndrome (22q11DS) is a neurodevelopmental disorder associated with cranial nerve anomalies and disordered oropharyngeal function, including pediatric dysphagia. Using the LgDel 22q11DS mouse model, we investigated whether sensory neuron differentiation in the trigeminal ganglion (CNgV), which is essential for normal orofacial function, is disrupted.
View Article and Find Full Text PDFDiurnal Control of Sensory Axon Growth and Shedding in the Mouse Cornea.
Invest Ophthalmol Vis Sci
September 2020
Purpose: The circadian clock plays an important role in the expression and regulation of various genes and cellular processes in the body. Here, we study diurnal regulation of the growth and shedding of the sensory axons in the mouse cornea.
Methods: Male and female BALB/cN mice were euthanized 90 minutes before and after the lights are turned on and off; at 5:30 AM, 8:30 AM, 5:30 PM, and 8:30 PM.