Role of CYP2D6 polymorphisms in tramadol metabolism in a context of co-medications and overweight.

Very few quantitative data exist on tramadol metabolites, which hampers our understanding of their role in efficacy and safety of tramadol. We aimed to provide quantitative data on tramadol and its 5 main metabolites in a patient cohort and to determine whether metabolite ratios can be predictive of a CYP2D6 metabolism phenotype. We also aimed to investigate the influence of co-medications and patient profile (BMI, glycemia, lipid levels) on tramadol metabolite ratios.

mARC1 Is the Main Contributor to Metabolic Reduction of -Hydroxyurea.

-Hydroxyurea has been known since the 1960s as an antiproliferative drug and is used both in oncology and for treatment of hematological disorders such as sickle cell anemia where very high daily doses are administered. It is assumed that the cellular effect of -hydroxyurea is caused by inhibition of ribonucleotide reductase, while alternative mechanisms, e.g.

Second-Coordination-Sphere Effects Reveal Electronic Structure Differences between the Mitochondrial Amidoxime Reducing Component and Sulfite Oxidase.

A combination of X-ray absorption and low-temperature electronic absorption spectroscopies has been used to probe the geometric and electronic structures of the human mitochondrial amidoxime reducing component enzyme (hmARC1) in the oxidized Mo(VI) and reduced Mo(IV) forms. Extended X-ray absorption fine structure analysis revealed that oxidized enzyme possesses a 5-coordinate [MoO(S)(PDT)] (PDT = pyranopterin dithiolene) active site with a cysteine coordinated to Mo. A 5-coordinate geometry is retained in the reduced state, with the equatorial oxo being protonated.

Photopatterning of conductive hydrogels which exhibit tissue-like properties.

Hydrogels are three-dimensional, highly tunable material systems that can match the properties of extracellular matrices. In addition to being widely used to grow and modulate cell behavior, hydrogels can be made conductive to further modulate electrically active cells, such as neurons, and even incorporated into multielectrode arrays to interface with tissues. To enable conductive hydrogels, graphene flakes can be mechanically suspended into a hydrogel precursor.

Long-term psycho-traumatic consequences of the COVID-19 health crisis among emergency department healthcare workers.

Assess the changes in post-traumatic stress disorder (PTSD), burnout, anxiety, depression, jobstrain, and isostrain levels over time among healthcare workers in emergency departments (EDs) after successive outbreaks of COVID-19. A prospective, multicenter study was conducted in 3 EDs and an emergency medical service. Healthcare workers who participated in our previous study were invited to participate in a follow-up 16 and 18 months and completed the questionnaires to assess symptoms of PTSD, burnout, anxiety, depression, jobstrain, and isostrain.