Publications by authors named "B A Cimini"
Understanding cellular responses to external stimuli is critical for parsing biological mechanisms and advancing therapeutic development. High-content image-based assays provide a cost-effective approach to examine cellular phenotypes induced by diverse interventions, which offers valuable insights into biological processes and cellular states. In this paper, we introduce MorphoDiff, a generative pipeline to predict high-resolution cell morphological responses under different conditions based on perturbation encoding.
View Article and Find Full Text PDFNeuropsychiatric conditions pose substantial challenges for therapeutic development due to their complex and poorly understood underlying mechanisms. High-throughput, unbiased phenotypic assays present a promising path for advancing therapeutic discovery, especially within disease-relevant neural tissues. Here, we introduce NeuroPainting, a novel adaptation of the Cell Painting assay, optimized for high-dimensional morphological phenotyping of neural cell types, including neurons, neuronal progenitor cells, and astrocytes derived from human stem cells.
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- Bioimage analysis (BIA) enhances biological research by providing objective, quantitative methods for microscopy, overcoming biases linked to subjective analysis.
- Establishing dedicated BIA support in academic institutions is essential for improving research quality and facilitating scientific advancements.
- The document outlines challenges facing BIA, such as lack of training and recognition, and proposes strategies for improvement, including better training resources, standardization of tools, and increased collaboration and funding.
Article Synopsis
- Widespread sequencing has identified thousands of missense variants linked to diseases, creating a challenge in assessing their functional impact at scale.
- A new high-throughput imaging platform was developed to evaluate the effects of 3,448 missense variants across over 1,000 genes, revealing that mislocalization of proteins is a frequent outcome.
- Mislocalization affects about one-sixth of pathogenic variants and is mainly caused by issues with protein stability and membrane insertion, which can influence disease severity and help interpret uncertain variants.
Image-based profiling has been used to analyze cell health, drug mechanism of action, CRISPR-edited cells, and overall cytotoxicity. Cell Painting is a broadly used image-based assay that uses morphological features to capture how cells respond to treatments. However, this method requires cell fixation for staining, which prevents examining live cells.
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