Optimization of fermentation parameters for high-activity inulinase production and purification from by Plackett-Burman and Box-Behnken.

The aim of study was to optimize fermentation parameters for inulinase production from by a statistical approach and to carry out purification of inulinase. Five isolated fungal strains were screen out inulin degradation by using Lugol's iodine solution. exhibited maximum zone of clearance around the colony and was used as an inulinase producer.

1,2-Diborolanes with strong donor substituents: Synthesis and high antimicrobial activity.

1,2-diborolanes with strong and without strong donor substituents have been described, and are also referred to as 1,2-diboracyclopentane. The 1,2-diaryl/alkyl-amino-1,2-diboracyclopentanes 2, 3, and 4 were obtained in good yield after the reaction of 1,2-dichloro-1,2-diboracyclopentane 1 with ArNHLi and MeSi-NR. The structures of these new derivatives were characterized by nuclear magnetic resonance spectroscopy.

Cloned Gene Confers Low Level Erythromycin but High Level Clindamycin Resistance in NZ131.

The most common macrolide resistance mechanisms in streptococci are the presence of methylase encoding genes and or the presence of efflux encoded by genes. In the present study we aimed to show the effects of the gene under isogenic conditions on the activities of macrolides and lincosamides in streptococci. Total DNA was extracted from C1, and the gene was amplified with or without the regulatory region using modified primer with insertion of restriction sites to clone in to pUC18.

Influence of the particle size and form of feed on growth performance, digestive tract traits and nutrient digestibility of white egg-laying pullets from 1 to 112 D of age.

The influence of the grinding and physical form of diets on growth performance, gastrointestinal tract (GIT) development and nutrient digestibility was studied in layer pullets from hatch to 112 d of age. Diet formed a 2 × 2 factorial with 2 hammer mill screen sizes to grind cereals (4 and 8 mm) and 2 forms (mash and crumble). A total of 864 1-day-old layer chickens were randomly assigned to 1 of the 4 treatment groups with 8 replicates (27 birds per replicate).

Synthesis, Characterization, and Biological Activity of N (')-[(Z)-(3-Methyl-5-oxo-1-phenyl-1,5-dihydro-4H-pyrazol-4-ylidene)(phenyl)methyl]benzohydrazide and Its Co(II), Ni(II), and Cu(II) Complexes.

Reaction of 1-phenyl-3-methyl-4-benzoyl-pyrazol-5-one and benzoyl hydrazide in refluxing ethanol gave N (')-[(Z)-(3-methyl-5-oxo-1-phenyl-1,5-dihydro-4H-pyrazol-4-ylidene)(phenyl)methyl]benzohydrazide (HL(1)), which was characterized by NMR spectroscopy and single-crystal X-ray structure study. X-ray diffraction analyses of the crystals revealed a nonplanar molecule, existing in the keto-amine form, with intermolecular hydrogen bonding forming a seven-membered ring system. The reaction of HL(1) with Co(II), Ni(II), and Cu(II) halides gave the corresponding complexes, which were characterized by elemental analysis, molar conductance, magnetic measurements, and infrared and electronic spectral studies.

Spectroscopic and biological approach of Ni(II), Cu(II) and Co(II) complexes of 4-methoxy/ethoxybenzaldehyde thiosemicarbazone glyoxime.

Two novel vicinal dioxime ligands containing (4-methoxybenzaldehyde thiosemicarbazone glyoxime (L(1)H2) or 4-ethoxybenzaldehyde thiosemicarbazone glyoxime (L(2)H2)) thiosemicarbazone units were synthesized and characterized using (1)H NMR, (13)C NMR, HMQC, MS, infrared and, UV-VIS. spectroscopy, elemental analysis, and magnetic susceptibility measurements. Mononuclear nickel(II), copper(II) and cobalt(II) complexes with a metal:ligand ratio of 1:2 for L(1)H2 and L(2)H2 were also synthesized.

Reversible immobilization of urease by using bacterial cellulose nanofibers.

In this work, bacterial cellulose nanofibers were produced by using the Gluconacetobacter hansenii HE1 strain. These nanofibers were derivatized with dye affinity ligand Reactive Green 5, and these newly synthesized dye-attached nanofibers were used for affinity adsorption of urease. Reactive Green 5-attached nanofibers were characterized by Fourier transform infrared spectroscopy, SEM, and energy-dispersive x-ray spectroscopy analysis.

Isolation and Characterization of a Bacteriocin-Like Substance Produced by Geobacillus toebii Strain HBB-247.

A total of 201 thermophilic bacteria isolated from various thermal spring, mud and soil were tested for their antibacterial activity. Among the mostly active isolates, Geobacillus toebii HBB-247 was further examined. Bacteriocin-like inhibitory substance (BLIS) produced by strain HBB-247 was found to be stable up to 60°C, sensitive to proteolytic enzymes and effective against Enterococcus faecalis, Listeria sp.

Biological decolorization of textile dyes from isolated microfungi.

In this study, biological decolorization of two textile dyestuff (Benazol black ZN and Cibacron black W-NN) was comparatively studied using 22 microfungi strains isolated from polluted industrial soil areas. The initial dye concentrations in the medium were 250 and 500 mg l(-1). Benazol black ZN was the best decolorized by Haematonectria haematococca (HH1) (36.

Phenotypic and genotypic characterization of bacteriocins in enterococcal isolates of different sources.

Unlabelled: A collection of 57 enterococcal isolates from different origin (including river, treatment plant, spring and garbage water, soil, animal, and vegetables from Aydın) was screened for the production of bacteriocins. Enterococci were identified at species levels as Enterococcus faecium (34), E. hirae (6), E.

The short-term effects of different doses of dexamethasone on the numbers of some bacteria in the ileum.

Glucocorticoids are known to affect intestinal biota both directly or indirectly. The aim of the study reported here was to determine the short-term effects of different doses of dexamethasone on the numbers of various ileal bacteria populations. Rats were randomly put into groups, and each group was administered a single-dose injection of dexamethasone at either 0.

Zygomycotic proventriculitis and ventriculitis in ostriches (Struthio camelus) with impaction.

Three young ostriches (Struthio camelus), aged 4 months, were found to have zygomycotic proventriculitis and ventriculitis associated with impaction. Clinical signs were anorexia, chronic weight loss, weakness and lethargy followed by scant faeces for seven days. Proventriculi and ventriculi from birds were full of masses of hay, grass, leaves and other fibrous materials in combination with sand, gravel and plastic.