Secretion of the human parathyroid hormone through a microcin type I secretion system in Escherichia coli.

Background: Gram negative bacteria possess different secretion systems to export proteins to the extracellular medium. The simplest one, type I secretion system (T1SS), forms a channel across the cell envelope to export proteins in a single step. Peptides secreted by the T1SSs comprise a group of antibiotics, called class II microcins, which carry an amino terminal secretion domain that is processed concomitantly with export.

Characterization of Streptococcus equi subsp. zooepidemicus isolates containing lnuB gene responsible for the L phenotype.

Within the framework of the β-hemolytic streptococci surveillance carried out by the National Reference Laboratory from Uruguay, three putative Streptococcus equi subsp. zooepidemicus (SEZ) were received from different health centers. Being these the first reports associated with human infections in Uruguay, the objective of this work was to confirm their identification, to determine their genetic relationship and to study their antibiotic susceptibility.

Nutrition, dietary recommendations, and supplements for patients with congenital heart disease.

Nutrition is the cornerstone of a healthy life and is crucial for the prevention of cardiovascular disease. Patients with congenital heart disease (CHD) are prone to nutrition disorders, including abnormalities in body composition such as overweight or obesity which, along with other classic cardiovascular risk factors, places our increasing and aging adult CHD (ACHD) population at a higher risk for acquired cardiovascular disease. These patients are also at risk of cachexia or sarcopenia as well as macronutrient and micronutrient deficiencies derived from the development of heart failure as a complication of their underlying cardiac disease.

Potential biomarkers of infertility associated with microbiome imbalances.

Problem: The aim of this study was to investigate the possible relationship between vaginal/rectal microbiome disbalances and miRNA expression with infertility.

Method Of Study: Observational, exploratory, preliminary study. A total of 287 multiple IVF failure infertile patients were recruited.

On sulfonamide resistance, sul genes, class 1 integrons and their horizontal transfer in Escherichia coli.

Class 1 integrons (Int1) contribute to antibiotic multiresistance in Gram-negative bacteria. Being frequently carried by conjugative plasmids, their spread would depend to some extent on their horizontal transfer to other bacteria. This was the main issue that was addressed in this work: the analysis of Int1 lateral transfer in the presence of different antibiotic pressures.

Analysis of RecA-independent recombination events between short direct repeats related to a genomic island and to a plasmid in K12.

RecA-independent recombination events between short direct repeats, leading to deletion of the intervening sequences, were found to occur in two genetic models in the K12 background. The first model was a small genomic island which had been shown to be mobile in its strain of origin and, when cloned, also in the K12 context. However, it did not encode a site-specific recombinase as mobile genomic islands usually do.

Probiotic activity of Enterococcus faecalis CECT7121: effects on mucosal immunity and intestinal epithelial cells.

Aims: To analyse the effect of Enterococcus faecalis CECT7121 on intestinal epithelial cells (IECs) and its effects on the mucosal immune response.

Methods And Results: Enterococcus faecalis CECT7121 showed a high adhesion capacity to completely and heterogeneously differentiated human intestinal epithelial cell line (Caco-2 cells). In addition, the contact of this bacterium with Caco-2 cells did not induce inflammatory chemokines (IL-8 and CCL-20).

Microcin H47 system: an Escherichia coli small genomic island with novel features.

Genomic islands are DNA regions containing variable genetic information related to secondary metabolism. Frequently, they have the ability to excise from and integrate into replicons through site-specific recombination. Thus, they are usually flanked by short direct repeats that act as attachment sites, and contain genes for an integrase and an excisionase which carry out the genetic exchange.

Preliminary studies on the prevention of the ovalbumin-induced allergic response by Enterococcus faecalis CECT7121 in mice.

Background: Allergic diseases are featured by an increased production of IgE due to an imbalance in the immune response towards a Th2 profile. In this work, the ability of Enterococcus faecalis CECT7121 to regulate this Th2-exaggerated response in a murine model of ovalbumin (OVA)-induced allergy was studied.

Methods: BALB/c mice intragastrically inoculated with E.

Beneficial activity of Enterococcus faecalis CECT7121 in the anti-lymphoma protective response.

Aims: To study the anti-tumour effects of Enterococcus faecalis CECT7121 on LBC cells, an aggressive murine T-cell lymphoma that kills the host in 18 days when is intraperitoneally (i.p.) administrated.

Microcins and urovirulence in Escherichia coli.

Urinary tract infections are among the most common infectious diseases encountered in humans and Escherichia coli is their leading etiologic agent. Uropathogenic E. coli encompasses a group of bacteria possessing a variable virulence gene assortment.

Modular structure of microcin H47 and colicin V.

Microcins are gene-encoded peptide antibiotics produced by enterobacteria that act on strains of gram-negative bacteria. In this work, we concentrated on higher-molecular-mass microcins, i.e.

Comparative analysis of chromosome-encoded microcins.

Microcins are ribosomally synthesized peptide antibiotics that are produced by enterobacterial strains. Although the first studies concentrated on plasmid-encoded activities, in the last years three chromosome-encoded microcins have been described: H47, E492, and M. Here, a new microcin, I47, is presented as a fourth member of this group.

Involvement of enterobactin synthesis pathway in production of microcin H47.

Microcin H47 (MccH47) is a gene-encoded peptide antibiotic produced by an Escherichia coli clinical isolate which is active on strains of gram-negative bacteria. Its uptake by E. coli K-12-susceptible cells depends on the presence of any of the outer membrane proteins Cir, Fiu, and FepA, the three catechol receptors of this organism.

The structure, function, and origin of the microcin H47 ATP-binding cassette exporter indicate its relatedness to that of colicin V.

Microcin H47, a gene-encoded peptide antibiotic produced by a natural Escherichia coli strain, was shown to be secreted by a three-component ATP-binding cassette exporter which was revealed to be strongly related to that of colicin V. The results of sequence and gene fusion analyses, as well as heterologous complementation assays, are presented.