Characterization and peptide identification of umami fractions from rusip-a traditional fermented anchovy product.

Rusip is a spontaneously fermented anchovy product originating from Bangka Belitung province in Indonesia, used as umami seasoning. This study investigated the characteristics of rusip and its umami fractions to reveal umami peptides in rusip. Six commercial rusip samples from Bangka were analyzed for their proximate composition, pH, total titratable acids, NaCl, total sugars, free glutamic acid, nucleotide (adenosine monophosphate), and total amino acid composition.

NMR fingerprinting of depolymerised durian rind pectin and its antibacterial activity.

This study investigated the effect of commercial sterilisation treatment (121 °C for 15 min) on durian rind pectin. The change of structure was observed by NMR (nuclear magnetic resonance) and the antibacterial activity was assessed by microdilution method to obtain the minimum inhibitory concentration (MIC) value. NMR spectra revealed the rhamnogalacturonan-I and homogalacturonan structure, with lower methyl-ester in sterilised pectin.

Continuous production of velvet bean-based bioactive peptides in membrane reactor with dual enzyme system.

One of the main challenges hindering the commercialization of bioactive peptides is the lack of scalable and consistent production methods. To overcome this obstacle, an automated enzyme membrane reactor was used to continuously produce bioactive peptides from velvet bean (Mucuna pruriens). The optimum operating conditions were [E]/[S] = 5%, pH = 7.

Production of Modified Nucleosides in a Continuous Enzyme Membrane Reactor.

Nucleoside analogues are important compounds for the treatment of viral infections or cancers. While (chemo-)enzymatic synthesis is a valuable alternative to traditional chemical methods, the feasibility of such processes is lowered by the high production cost of the biocatalyst. As continuous enzyme membrane reactors (EMR) allow the use of biocatalysts until their full inactivation, they offer a valuable alternative to batch enzymatic reactions with freely dissolved enzymes.

Enzymatic Synthesis of Human Milk Fat Substitute - A Review on Technological Approaches.

Human milk fat substitute (HMFS) is a structured lipid designed to resemble human milk fat. It contains 60-70% palmitic acid at the -2 position and unsaturated fatty acids at the -1,3 positions in triacylglycerol structures. HMFS is synthesized by the enzymatic interesterification of vegetable oils, animal fats or a blend of oils.

Increased γ-Aminobutyric Acid Content of Germinated Brown Rice Produced in Membrane Reactor.

Research Background: Rice germination is a natural approach to enhance the physical and functional properties of brown rice. Thus, the aim of this study is to investigate the influence of different germination methods on functional properties of germinated brown rice and evaluate the process feasibility.

Experimental Approach: Brown rice of IPB 3S variety was germinated with three different methods: () complete soaking without water replacement, () complete soaking with water replacement every six hours, and () complete soaking with continuous washing in the developed membrane-facilitated soaking reactor.

Enzymatic Preparation of Bioactive Peptides Exhibiting ACE Inhibitory Activity from Soybean and Velvet Bean: A Systematic Review.

The Angiotensin-I-converting enzyme (ACE) is a peptidase with a significant role in the regulation of blood pressure. Within this work, a systematic review on the enzymatic preparation of Angiotensin-I-Converting Enzyme inhibitory (ACEi) peptides is presented. The systematic review is conducted by following PRISMA guidelines.

The utilization of bilimbi () and lime () juices as natural acid coagulants for tofu production.

The influences of coagulation conditions on the characteristics of tofu have been investigated by many studies, with limited perspectives on the utilization of organic acid coagulants. Hence, this research aimed to study the psychochemical and functional properties of tofu coagulated by bilimbi () and lime () juices. The highest tofu protein content was quantified for tofu prepared with 20% bilimbi and 5% lime juices, as much as 52.

Membrane-based preparative methods and bioactivities mapping of tempe-based peptides.

This study was aimed to produce bioactive peptides from optimally fermented tempe, and map their overall bioactivities. There were three preparative methods utilized for producing tempe-based peptides, such as water-facilitated extraction, alcalase, and papain hydrolysis, and in combination with membrane filtration. Fermenting soybean at 144 h was selected as the optimum time based on protein content and degree of hydrolysis.

Lipase-Catalyzed Interesterification for the Synthesis of Medium-Long-Medium (MLM) Structured Lipids - A Review.

Medium-long-medium (MLM) structured lipids typically contain medium-chain fatty acids (C6-C12) at -1,3 and long-chain fatty acids (C14-C24) at -2 positions. They have reduced calories and are suitable for the control of obesity, lipid malabsorption and other metabolic disorders. This review focuses on the synthesis of MLM lipids by the enzymatic interesterification.

Recent advances on prebiotic lactulose production.

Lactulose, a synthetic disaccharide, has received increasing interest due to its role as a prebiotic. The production of lactulose is important in the dairy industry, as it is regarded as a high value-added derivative of whey or lactose. The industrial production of lactulose is still mainly done by chemical isomerization.

Influences of operating conditions on continuous lactulose synthesis in an enzymatic membrane reactor system: A basis prior to long-term operation.

Lactulose synthesis was performed in a continuous stirred enzymatic membrane reactor. Each investigated operating condition (agitation, pH, feed molar ratio of lactose to fructose (mL/mF ratio), hydraulic residence time (HRT)) had an influence on reaction performances, in terms of lactulose concentration, productivity and selectivity. Lactulose concentration was maximum at an mL/mF ratio of 1/2.

Continuous synthesis of lactulose in an enzymatic membrane reactor reduces lactulose secondary hydrolysis.

Newly developed parallel small-scale enzymatic membrane reactors (EMRs) were used to enhance the synthesis of lactulose using β-galactosidase. Under batch operation, the productivity of lactulose decreased abruptly from 2.72 down to 0.

Effect of pellet size and stimulating factor on the glucosamine production using Aspergillus sp. BCRC 31742.

The higher GlcN production using a wild-type fungi, Aspergillus sp. BCRC 31742 cultivated under submerged fermentation was investigated. Several fermentation aspects were studied, such as pellet size, working volume, agitation rate and stimulating factor.