Fusion of a chitin-binding domain to an antibacterial peptide to enhance resistance to in tobacco ().

An antibacterial peptide-encoding gene from alfalfa seeds, alfAFP, was fused to the C-terminal part of chitin-binding domain (CBD) of the rice chitinase-encoding gene (CBD-alfAFP) and introduced to tobacco by -mediated transformation. Polymerase chain reaction (PCR) technique was used to confirm the integration of the recombinant CBD-alfAFP encoding gene in transgenic tobacco plants. A number of transgenic lines and a non-transgenic control plant were selected for further molecular analyses.