Synthesis, spectral characterization, lethal dose (LD) and acute toxicity studies of 1,4-Bis(imidazolylazo)benzene (BIAB).

The preparation and spectral identification of new heterocyclic azo ligand 1,4-Bis(imidazolylazo)benzene (BIAB) was prepared by reacting a diazonium chloride salt solution of 1,4-diaminobenzene with imidazole in alkaline ethanolic solution. Differing spectral techniques have been used to study the structure of the azo dye ligand (BIAB) such as Elemental analysis (C.H.