Publications by authors named "Azaiez Sana"

Enterobacterales resistant to extended-spectrum cephalosporins (ESC) are a marker of the antimicrobial resistance (AMR) burden. They are infecting humans, but the intestinal microbiota can also be transiently colonized without developing symptoms. Healthy carriage can promote silent dissemination of resistant bacteria, and data on this colonization are often lacking.

View Article and Find Full Text PDF
Article Synopsis
  • * In a study conducted in Monastir, Tunisia, despite official monitoring indicating compliant water quality, 31 samples (19.1%) contained ESC-resistant bacteria, with 39 isolates from E. coli, Klebsiella pneumoniae, and Citrobacter freundii identified.
  • * The findings revealed a consistent presence of resistant bacteria across different sites, suggesting that improvements in water quality monitoring could be achieved by including tests for total coliforms and ESBL counts on selective
View Article and Find Full Text PDF

Objectives: Wild birds are vectors of antimicrobial resistance. Birds living in close contact with humans or other animals, like feral pigeons (Columba livia), might be especially prone to acquire resistance genes such as those encoding extended-spectrum beta-lactamases (ESBLs) and carbapenemases.

Methods: Cloacal samples (n = 206) of free-living feral pigeons (C.

View Article and Find Full Text PDF

Carbapenem-resistant (CRAB) strains can cause severe and difficult-to-treat infections in patients with compromised general health. CRAB strains disseminate rapidly in nosocomial settings by patient-to-patient contact, through medical devices and inanimate reservoirs. The occurrence of CRAB in patients residing in the intensive care units (ICUs) of the Sahloul University hospital in Sousse, Tunisia is high.

View Article and Find Full Text PDF

European starlings are widespread migratory birds that have already been described as carrying bacteria resistant to extended-spectrum cephalosporins (ESC-R). These birds are well known in Tunisia because they spend the wintertime in this country and are hunted for human consumption. The goal of our study was to estimate the proportion of ESC-R in these birds and to characterize the collected isolates using whole-genome sequencing.

View Article and Find Full Text PDF

Resistance to extended-spectrum cephalosporins (ESC) and carbapenems in Enterobacterales is a major issue in public health. Carbapenem resistance in particular is associated with increased morbidity and mortality. Moreover, such resistance is often co-harbored with resistance to non-beta-lactam antibiotics, and pathogens quickly become multi-drug-resistant (MDR).

View Article and Find Full Text PDF
Article Synopsis
  • The gut microbiota significantly impacts metabolism, immune function, and the nervous system, making its study crucial for understanding health and disease.
  • This study characterizes the gut microbiota of healthy Tunisian adults using 16S rRNA gene sequencing, focusing on differences based on gender and body mass index (BMI).
  • Results show that the Tunisian gut microbiota is primarily composed of Firmicutes and Bacteroidota, with notable differences related to gender and weight, establishing a baseline for future health assessments in Tunisia.
View Article and Find Full Text PDF
Article Synopsis
  • A study evaluated 450 bacterial strains for their ability to combat bacterial soft rot in potatoes caused by Pectobacterium carotovorum, identifying strain Ar10 as a strong antagonist.
  • Strain Ar10, identified as Bacillus amyloliquefaciens, produced a cell-free supernatant with significant antibacterial activity against various bacteria, showing a killing rate of up to 96% against P. carotovorum.
  • Treatment of potato tubers with strain Ar10 or its supernatant significantly reduced disease symptoms, indicating that the glycolipid compound produced by Ar10 could be a promising alternative for managing potato soft rot.
View Article and Find Full Text PDF

A protease-producing strain CT2 isolated from Tunisian potatoes, exhibiting a potent protease activity (prot CT2), was identified as Bacillus halotolerans according to 16S ribosomal DNA sequence analysis. Maximum prot CT2 production was obtained in medium supplemented with bean seed proteins. Proteolytic activity was purified by ammonium sulphate precipitation, Sephacryl S-200 gel filtration and SP-sepharose cation-exchange chromatography.

View Article and Find Full Text PDF

A strain producing chitinase, isolated from potato stem tissue, was identified as Bacillus licheniformis by biochemical properties and 16S RNA sequence analysis. Statistical experimental designs were used to optimize nine independent variables for chitinase production by B. licheniformis AT6 strain in submerged fermentation.

View Article and Find Full Text PDF

Background: Acute coronary syndromes (ACS) are complex and polygenic diseases which are a real problem of public health. These syndromes require multidisciplinary studies to understand the pathogenesis mechanisms. Our study aims to evaluate the endothelin-1 (ET-1) serum concentration in Tunisian coronary compared to controls healthy, as well as the study of the impact of an intronic polymorphism A (8002) G of pre-pro-endothelin-1 Gene (inactive precursor of ET-1) on the change in serum endothelin-1 and in the susceptibility to Acute coronary syndrome (SCA).

View Article and Find Full Text PDF

In the present study, the synergism of the lipopeptide bacillomycin D in combination with the polyene amphotericin B against pathogenic Candida species is described along with their potential cytotoxicity against mammalian cells. Bacillomycin D inhibited the growth of various Candida species at minimal concentrations from 12.5 to 25 μg ml(-1).

View Article and Find Full Text PDF
Article Synopsis
  • Researchers isolated endophyte bacteria from olive tree leaves that produce volatile organic compounds (VOCs) and tested their antifungal properties against harmful fungi.
  • The strain identified as P2, related to Pseudomonas sp., demonstrated significant antifungal activity, inhibiting the growth of Rhizoctonia solani by 86% and reducing fungal infections in potato slices.
  • Microscopy revealed that P2 alters the structure of R. solani, and VOC analysis identified key compounds like dimethyl disulfide and dimethyl trisulfide, indicating P2's potential as a protective agent for tuber crops against fungal diseases.*
View Article and Find Full Text PDF

A PHP Error was encountered

Severity: Warning

Message: fopen(/var/lib/php/sessions/ci_sessionrei8dg70lp29pufth13ueghsqk396jgg): Failed to open stream: No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 177

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)

Filename: Session/Session.php

Line Number: 137

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once