Publications by authors named "Ayumi Nakanishi"
The post-synthetic modification of an oligonucleotide is a powerful strategy for the synthesis of various analogs of the oligonucleotide, aiming to achieve the desired functions. In this study, we synthesized the thymidine phosphoramidite of 2'--pentafluorophenoxycarbonyl-2'-amino-LNA, which was introduced into oligonucleotides. Oligonucleotides containing a 2'--pentafluorophenoxycarbonyl-2'-amino-LNA unit could be isolated under ultra-mild deprotection conditions (50 mM KCO in MeOH at room temperature for 4 h).
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- Reactive oxygen species trigger neuronal cell death, but the specific processes behind this phenomenon remain unclear, particularly concerning neurite degeneration prior to cell death.
- In this study, the calcium ionophore ionomycin was used to induce cell death and neurite degeneration in neuroblastoma N1E-115 cells, showing effects that depend on its concentration and exposure time.
- Results indicated that ionomycin causes a significant increase in calcium influx and mitochondrial lipid hydroperoxide production, contributing to neurite degeneration, with lower reactive oxygen species emissions detected in the neurites compared to the cell body.