Publications by authors named "Ayten Kalfe"
The complex cell metabolism and its link to oncogenic signaling pathways have received huge interest within the last few years. But the lack of advanced analytical tools for the investigation of living cell metabolism is still a challenge to be faced. Therefore, we designed and fabricated a novel miniaturized microslot NMR detector with on-board heater integrated with a microfluidic device as NMR sample holder.
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- Developed a microfluidic device for detecting pathogens by isolating and amplifying low concentrations of eukaryotic mRNA, specifically targeting hsp70 mRNA from Cryptosporidium parvum.
- Achieved optimal mRNA isolation and amplification by modifying the PMMA channel surface with carboxylation and immobilizing PAMAM dendrimers, enhancing the effectiveness of oligo(dT)25 without needing additional blocking agents.
- Successfully demonstrated on-chip isolation and amplification of mRNA from as few as 30 C. parvum oocysts, showing this microfluidic approach outperformed traditional benchtop methods.