Modeling for Smart Vaccination against Peste des Petits Ruminants (PPR) in the Emirate of Abu Dhabi, United Arab Emirates.

Peste des petits ruminants (PPR) is a contagious and economically important transboundary viral disease of small ruminants. The United Arab Emirates (UAE) national animal health plan aimed to control and eradicate PPR from the country by following the global PPR control and eradication strategy which adopts small ruminants' mass vaccination to eradicate the disease from the globe by 2030. A smart vaccination approach, which is less expensive and has longer-term sustainable benefits, is needed to accelerate the eradication of PPR.

DNA-free high-quality RNA extraction from 39 difficult-to-extract plant species (representing seasonal tissues and tissue types) of 32 families, and its validation for downstream molecular applications.

Background: High-purity RNA serves as the basic requirement for downstream molecular analysis of plant species, especially the differential expression of genes to various biotic and abiotic stimuli. But, the extraction of high-quality RNA is usually difficult from plants rich in polysaccharides and polyphenols, and their presence usually interferes with the downstream applications. The aim of the study is to optimize the extraction of high-quality RNA from diverse plant species/tissues useful for downstream molecular applications.