A New Buffer for Gallium-68 Labeling Suitable for use in Nuclear Medicine; Triethanolamine (TEA).

Background: Radiopharmaceuticals labeled with [Ga] from positron emission tomography (PET) radionuclides are utilized in nuclear medicine for non-invasive in vivo molecular imaging. Buffer solutions for radiolabeling play an important role as choosing the right buffer for the reaction helps to obtain high yield radiopharmaceuticals. Zwitterionic organic buffer 4-(2- hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), sodium acetate (CHCOONa), sodium bicarbonate (NaHCO) buffers are widely used for labeling of peptides with [Ga]Cl.

Comparison of three different methods in [ Ga]Ga-PSMA11 radiolabeling.

In this study, quality control parameters such as radiochemical yield, radiochemical purity, and in vitro stability of gallium ( Ga)-prostate-specific membrane antigen-11 ([ Ga]Ga-PSMA11) radiopharmaceutical obtained in a research laboratory with three different synthesis algorithms were evaluated and compared. Gallium ( Ga) chloride precursor to be used in labeling in all three methods was obtained by using ITG brand Ge/ Ga generator. The first method for the [ Ga]Ga-PSMA11 radiopharmaceutical was performed with the automated synthesis module, which is widely used in clinical practice.

High-Efficiency Cationic Labeling Algorithm of Macroaggregated Albumin with Gallium.

Purpose: The generator product radionuclide gallium-68(Ga) is widely used for PET/CT imaging agents and the Ga-labeled MAA is an attractive alternative to Tc-labeled MAA. Using a commercially available MAA labeling kit for Tc, we presented a reliable synthesis protocol with a highly efficient, organic solvent-free cationic method in GMP conditions in the Scintomics automated synthesis unit.

Methods: The labeling process was performed by incubating for 7 min at 90 C in the borax vial containing the generator product GaCl MAA-HEPES eluted from the PSH cartridge with 1.

Validation of HPLC method for the determination of chemical andradiochemical purity of a Ga-labelled EuK-Sub-kf-(3-iodo-y-) DOTAGA.

The prostate-specific membrane antigen (PSMA) represents an ideal biomarker for molecular imaging. Various PSMA-targeted radioligands are available for prostate cancer imaging. In this study, labeling of PSMA I&T with Ga, as well as validation of the radiochemical purity of the synthesis product by reverse phase radio high-performance liquid chromatography (HPLC) method are intended.

Comparison of Radiochemical and Chemical Impurities in Liquid Wastes of Two Different Ge/Ga Generators used in Nuclear Medicine PET Chemistry.

Objectives: Germanium-68/gallium-68 (Ge/Ga) generator eluate contains a number of metal cations that can compete with GaCl, reducing specific radioactivity. The first step in peptide labeling with GaCl is to remove Ge and several other metals with a long half-life. In this purification step, the elution residue that is passed through the cartridge is collected in glass waste bottles.

Ga-citrate positron emission tomography/computed tomography findings in an experimental model of acute appendicitis in rabbits.

Objective: Acute appendicitis (AA) is a common urgent surgical situation of the gastrointestinal tract. Gallium-68 (Ga)-citrate has been recently investigated as a radiopharmaceutical for infection and inflammation imaging. Aim of the study was to determine the effectiveness of Ga-citrate positron emission tomography/computed tomography (PET/CT) imaging in rabbits with experimentally induced AA.

Examination of metallic impurities of 68Ge/68Ga generators used for radioactive labeling of peptides in clinical PET applications.

Background/aim: Especially suitable for PET due to its nuclear physical and radiochemical properties, the positron emitter Gallium-68 (Ga) occurs by electron capture from Germanium-68 (Ge). In such a radionuclide generator, the germanium is bound to an insoluble, inert column matrix and forms a secular radioactive balance with 68Ga obtained in the hour. As a result of the limited radiochemical selectivity of the elution process, the eluate obtained is basically contaminated with the main nuclide traces, so that the eluate becomes a mixture of Ga and Ge radionuclides.

Detection of Pulmonary Embolism with Gallium-68 Macroaggregated Albumin Perfusion PET/CT: An Experimental Study in Rabbits.

This study was designed to evaluate the accuracy of detecting pulmonary embolism (PE) using the Technegas SPECT/CT combined with Ga PET/CT in a rabbit model. One hour after artificial PE ( = 6) and sham ( = 6) models were created, Technegas SPECT/CT ventilation and Ga-MAA PET/CT perfusion scan (V/Q scan) were performed. Ventilation imaging was performed first on all cases.

Physiological Animal Imaging with Ga-Citrate.

Background: Gallium-68 is an ideal research and hospital-based PET radioisotope. The uptake mechanism of Gallium citrate is a combination of specific and non-specific processes, for example, vasodilatation, increased vascular permeability, plasma transferrin binding and lactoferrin and siderophores.

Objective: In this study, by applying the Ge/Ga generator product, a simple technique for the synthesis and quality control of 68Ga-citrate was introduced and was followed by preliminary animal studies.

Assessment of Antibacterial Activity and Cytotoxicity Effect of Oil.

Background: Methicillin resistance is a serious health concern since it has spread among and coagulase-negative (CoNS) that are frequent community and nosocomial pathogens worldwide. Methicillin-resistant strains are often resistant to other classes of antibiotics, making their treatment difficult. oil is known to be active against Gram-positive cocci, yet its cytotoxicity is rarely investigated, is a proper and powerful candidate for treatment of methicillin-resistant isolates.

Investigation of the inflammatory biomarkers of metabolic syndrome in adolescents.

Background: Metabolic syndrome (MetS) is a chronic and multifactorial syndrome characterized by a low-grade chronic inflammation, and a major risk factor for type 2 diabetes mellitus (T2DM) and cardiovascular disease (CVD). In our study, we aimed to investigate the serum levels of high sensitive C-reactive protein (hs-CRP), haptoglobin (Hp), α2-macroglobulin (α2-MG), platelet factor-4 (PF-4), fetuin-A, serum amyloid P (SAP) and α1-acid glycoprotein (AGP) in an adolescent population with MetS.

Methods: This study was performed in 43 (18 males, 25 females) MetS adolescents between the ages of 13 and 17 years (14.

Late-onset Streptococcus pasteurianus sepsis in a preterm baby in a neonatal intensive care unit.

Apnea, cyanosis, lethargy and prolongation in capillary filling time developed on the postnatal 37(th) day in a preterm baby who was born at the 30(th) gestational week with a birth weight of 1 300 g. Acute phase reactants and immature/total neutrophil count ratio were found to be high. The patient who was diagnosed with sepsis was successfully treated with meropenem which was started empirically.