Effect of temperature and distance on the viral outcome of clinical specimens from acute flaccid paralysis cases in Nigeria.

Aim: The effect of cold box temperature and distance on virus titers, poliovirus isolation rate, and appearance of orphan polioviruses was investigated.

Methods: Cold boxes with stools were randomly selected and examined for internal temperature over a 7-month period. After virus isolation in Rhabdomyosarcoma (RD) cell line RDand L20B, titer calculations and intratypic differentiation were done on isolates.