Publications by authors named "Ayesha M Ahmed"
Article Synopsis
- Researchers have developed a new method called ORBIT, which allows for efficient manipulation of the E. coli genome using DNA oligonucleotides as opposed to traditional methods that are often low in efficiency and scalability.
- This approach enables precise knockouts and integrations of large genome fragments, significantly outperforming existing techniques.
- ORBIT can also create extensive mutant libraries by employing pools of targeting oligonucleotides, demonstrating its capability to scale up to over 30,000 unique genetic variations.
The scavenger receptor SR-BI plays an important role in the hepatic clearance of HDL cholesterol and other lipids, driving reverse cholesterol transport and contributing to protection against atherosclerosis in mouse models. We characterized the role of endocytosis in lipid uptake from HDL, mediated by the human SR-BI, using a variety of approaches to inhibit endocytosis, including hypertonic shock, potassium or energy depletion and disassembly of the actin cytoskeleton. Our studies revealed that unlike mouse SR-BI, human SR-BI-mediated HDL-lipid uptake was reduced by inhibition of endocytosis.
View Article and Find Full Text PDFScavenger receptor, class B, type I (SR-BI) mediates binding and internalization of a variety of lipoprotein and nonlipoprotein ligands, including HDL. Studies in genetically engineered mice revealed that SR-BI plays an important role in HDL reverse cholesterol transport and protection against atherosclerosis. Understanding how SR-BI's function is regulated may reveal new approaches to therapeutic intervention in atherosclerosis and heart disease.
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