Proteomics analysis of porcine endometrial cell-derived extracellular vesicles involved in embryo attachment.

Maternal-embryo interactions play a critical role in early mammalian development, with extracellular vesicles (EVs) playing a key role in intercellular communication. Recent studies have focused on the mechanisms by which maternal-derived factors, such as RNA, proteins, and metabolites influence gap junctions, EVs, and direct cell-to-cell interactions, contributing to embryonic development. In this study, using a proteomics approach, we investigated the impact of EVs secreted from porcine endometrial cells (pEECs) and their protein cargoes on embryonic development.

Effect of extracellular vesicles derived from oviductal and uterine fluid on the development of porcine preimplantation embryos.

To improve the efficiency of in-vitro-produced (IVP) porcine embryos, we focused on the events that usually occur during in-vivo embryonic transit from the oviduct to the uterus. Extracellular vesicles (EVs) are released by different mammalian cells and are imperative for intercellular communication and reflect the cell's physiological state. Based on these characteristics, EVs were isolated from oviductal and uterine fluid to imitate the in vivo environment and improve the efficiency of IVP embryos.

Embryotrophic effect of exogenous protein contained adipose-derived stem cell extracellular vesicles.

Background: Extracellular vesicles (EVs) regulate cell metabolism and various biological processes by delivering specific proteins and nucleic acids to surrounding cells. We aimed to investigate the effects of the cargo contained in EVs derived from adipose-derived stem cells (ASCs) on the porcine embryonic development.

Methods: ASCs were isolated from porcine adipose tissue and characterized using ASC-specific markers via flow cytometry.

Melatonin and resveratrol alleviate molecular and metabolic toxicity induced by Bisphenol A in endometrial organoids.

Bisphenol A (BPA), a widespread environmental contaminant, poses concerns due to its disruptive effects on physiological functions of the uterine endometrium. In contrast, melatonin (MT) and Resveratrol (RSV) are under scrutiny for their potential protective roles against BPA-induced damage. For the efficacy and ethical concerns in the animal test, endometrial organoids, three-dimensional models mimicking endometrium, serve as crucial tools for unraveling the impact of environmental factors on reproductive health.

Generation of porcine endometrial organoids and their use as a model for enhancing embryonic attachment and elongation.

In Brief: Porcine endometrial organoids (EOs) were isolated and characterized, revealing distinctive features such as unique extracellular matrix formation, fusion into uterine bud-like structures, and facilitation of embryo elongation. The yield of EOs was significantly enhanced by cryopreservation medium supplemented with the rock inhibitor (Y-27632), resulting in reduced expression of apoptotic mRNAs and microRNAs.

Abstract: Endometrial organoids (EOs) are acceptable models for understanding maternal-embryonic cross talk.

Effects of extracellular vesicles derived from steroids-primed oviductal epithelial cells on porcine in vitro embryonic development.

Extracellular vesicles (EVs) play an active role in regulating different physiological events, however, endocrine control of EVs cargo contents remain poorly understood. In this study, we aimed to isolate EVs from the porcine oviductal epithelial cells (POECs) that were primed with steroid hormones including estradiol (E) and progesterone (P), mimicking the in vivo conditions of the reproductive cycle and studied their effects on in vitro produced embryonic development. For this purpose, POECs were treated either with 0 concentration (control) or two different combinations of E and P including 50 pg/mL E + 0.