Untangling Zebrafish Genetic Annotation: Addressing Complexities and Nomenclature Issues in Orthologous Evaluation of TCOF1 and NOLC1.

Treacher Collins syndrome (TCS) is a genetic disorder affecting facial development, primarily caused by mutations in the TCOF1 gene. TCOF1, along with NOLC1, play important roles in ribosomal RNA transcription and processing. Previously, a zebrafish model of TCS successfully recapitulated the main characteristics of the syndrome by knocking down the expression of a gene on chromosome 13 (coding for Uniprot ID B8JIY2), which was identified as the TCOF1 orthologue.

Nitrate increases the capacity of an aerobic moving-bed biofilm reactor (MBBR) for winery wastewater treatment.

We used bench-scale tests and mathematical modeling to explore chemical oxygen demand (COD) removal rates in a moving-bed biofilm reactor (MBBR) for winery wastewater treatment, using either urea or nitrate as a nitrogen source. With urea addition, the COD removal fluxes ranged from 34 to 45 gCOD/m-d. However, when nitrate was added, fluxes increased up to 65 gCOD/m-d, twice the amount reported for aerobic biofilms for winery wastewater treatment.

A steady-state pH-control model for the biological production of elemental sulfur from sulfate in mining-influenced water.

We developed a model to predict pH, alkalinity, and the Langelier Saturation Index (LSI) in coupled systems of hydrogen-based autotrophic sulfate reduction and aerobic oxidation of sulfide to elemental sulfur. To neutralize the biologically generated base, the model allows for the addition of CO as part of the gas mixture, the independent addition of HCl or CO, or a combination of the alternatives. The model was evaluated against the results from a laboratory system for the production of elemental sulfur from sulfate present in mine-tailings water, which is characterized by the presence of elevated sulfate and calcium concentrations.

as a Clean Label Ingredient to Produce Nutritionally Enriched Food Emulsions.

flour, a sustainable source of protein and bioactive compounds, was used as a clean label ingredient in order to reformulate a commercial hummus sauce, replacing egg yolk and modified starch, improving its nutritional quality. For this purpose, the impact of different concentrations of insect flour on the sauce was studied. Rheology properties, texture profile analysis, and the microstructure of the sauces were analyzed.

High-Rate Sulfate Removal Coupled to Elemental Sulfur Production in Mining Process Waters Based on Membrane-Biofilm Technology.

It is anticipated that copper mining output will significantly increase over the next 20 years because of the more intensive use of copper in electricity-related technologies such as for transport and clean power generation, leading to a significant increase in the impacts on water resources if stricter regulations and as a result cleaner mining and processing technologies are not implemented. A key concern of discarded copper production process water is sulfate. In this study we aim to transform sulfate into sulfur in real mining process water.

The crucial role of model systems in understanding the complexity of cell signaling in human neurocristopathies.

Animal models are useful to study the molecular, cellular, and morphogenetic mechanisms underlying normal and pathological development. Cell-based study models have emerged as an alternative approach to study many aspects of human embryonic development and disease. The neural crest (NC) is a transient, multipotent, and migratory embryonic cell population that generates a diverse group of cell types that arises during vertebrate development.

Shear wave elastography in optic neuritis: diagnostic accuracy of the optic nerve and adjacent fat tissue values.

In this study, we attempt to determine the diagnostic performance of shear wave elastography of the optic nerve and adjacent fat tissue in patients with optic neuritis. The study included a patient group consisting of 72 eyes of 36 patients who were diagnosed with unilateral optic neuritis, and an age-matched control group of 36 eyes of 18 healthy subjects. The patient group consisted of 25 multiple sclerosis patients and 11 recurrent isolated optic neuritis patients.

Assessment of the Rigidity Changes of Corpus Cavernosum Penis in Vasculary Erectile Dysfunction (ED) Subtypes by Shear Wave Elastography (SWE).

Introduction: Erectile dysfunction (ED) is a condition that is very common all over the world, concerns about men and causes very important social problems. The aim of this study is to perform quantitative penile corpus cavernosum (CC) stiffness measurements by using SWE for patients with both types of vascular ED.

Methods: In our study, we handled the data of 101 participants in total.

Rhodotorula glutinis T13 as a potential source of microbial lipids for biodiesel generation.

Single cell oils (SCO) are a promising source of oils that could be exploited in different industrial areas. SCO for biodiesel production circumvents the controversy food vs. fuel, does not require large land areas for culture, and is independent of climate and seasonal variations, among other advantages in comparison to vegetable oils.

Geoffroea decorticans fruit extracts inhibit the wnt/β-catenin pathway, a therapeutic target in cancer.

Geoffroea decorticans (chañar) is commonly used for culinary and medicinal purposes in rural communities. The aim of this work was to chemically characterize three Geoffroea decorticans extracts and determine their capacity to modulate the wnt/β-catenin pathway. This signaling pathway plays a key role in embryonic development but its overactivation leads to cancer cell growth.

Neurogenesis From Neural Crest Cells: Molecular Mechanisms in the Formation of Cranial Nerves and Ganglia.

The neural crest (NC) is a transient multipotent cell population that originates in the dorsal neural tube. Cells of the NC are highly migratory, as they travel considerable distances through the body to reach their final sites. Derivatives of the NC are neurons and glia of the peripheral nervous system (PNS) and the enteric nervous system as well as non-neural cells.

A membrane-biofilm system for sulfate conversion to elemental sulfur in mining-influenced waters.

A system of two membrane biofilm reactors (MBfRs) was tested for the conversion of sulfate (1.5 g/L) in mining-process water into elemental sulfur (S) particles. Initially, a H-based MBfR reduced sulfate to sulfide, and an O-based MBfR then oxidized sulfide to S.

Testicular stiffness in varicocele: evaluation with shear wave elastography.

Purpose: This study was conducted to evaluate the potential role of shear wave elastography (SWE) in the evaluation of testes in patients with varicocele.

Methods: A total of 116 testes of 58 patients with left-sided varicocele and 58 testes of 29 age-matched healthy controls were included in the study. The patients' testes were classified into groups A (normospermic) and B (oligospermic) based on the presence of oligospermia.

Growth and lipid production of Rhodotorula glutinis R4, in comparison to other oleaginous yeasts.

Some Rhodotorula spp. have been characterized as oleaginous yeasts. Under certain culture conditions they can accumulate neutral lipids, which are mainly triglycerides (TAG).

Fatty acids profiles and estimation of the biodiesel quality parameters from Rhodotorula spp. from Antarctica.

Objective: Oleaginous yeasts are a renewable and alternative source of oil for third-generation biodiesel. This work aimed to evaluate the effects of glucose concentration (30-100 g L) on growth, lipid synthesis, and fatty acids (FA) profile of three Rhodotorula spp. (R.

The role of teratogens in neural crest development.

The neural crest (NC), discovered by Wilhelm His 150 years ago, gives rise to a multipotent migratory embryonic cell population that generates a remarkably diverse and important array of cell types during the development of the vertebrate embryo. These cells originate in the neural plate border (NPB), which is the ectoderm between the neural plate and the epidermis. They give rise to the neurons and glia of the peripheral nervous system, melanocytes, chondrocytes, smooth muscle cells, odontoblasts and neuroendocrine cells, among others.

Influence of operating conditions on sulfate reduction from real mining process water by membrane biofilm reactors.

Two H-based membrane biofilm reactor (H-MBfR) systems, differing in membrane type, were tested for sulfate reduction from a real mining-process water having low alkalinity and high concentrations of dissolved sulfate and calcium. Maximum sulfate reductions were 99%, with an optimum pH range between 8 and 8.5, which minimized any toxic effect of unionized hydrogen sulfide (HS) on sulfate-reducing bacteria (SRB) and calcite scaling on the fibers and in the biofilm.

Activation of and in Transgenic Mouse Embryonic Stem Cells Increases Differentiation into Neural Crest Derivatives.

The neural crest (NC) comprises a multipotent cell population that produces peripheral neurons, cartilage, and smooth muscle cells, among other phenotypes. The participation of and when expressed in mouse embryonic stem cells (mESCs) undergoing NC differentiation is unexplored. In this work, we generated stable mESCs transfected with constructs encoding chimeric proteins in which the ligand binding domain of glucocorticoid receptor (GR), which is translocated to the nucleus by dexamethasone addition, is fused to either (HGR) or (MGR), as well as double-transgenic cells (HGR+MGR).

Predation creates unique void layer in membrane-aerated biofilms.

The membrane-aerated biofilm reactor (MABR) is a novel wastewater treatment technology based on oxygen-supplying membranes. The counter diffusion of oxygen and electron donors in MABRs leads to unique behavior, and we hypothesized it also could impact predation. We used optical coherence tomography (OCT), microsensor analyses, and mathematical modeling to investigate predation in membrane-aerated biofilms (MABs).

Molecular characterization of wdr68 gene in embryonic development of Xenopus laevis.

WDR68, also known as DCAF7, is a WD40 repeated domain protein highly conserved in eukaryotic organisms in both plants and animals. This protein participates in numerous cellular processes and exerts its function through interaction with other proteins. In the present work, we isolated, sequenced and characterized cDNA corresponding to the wdr68 gene in embryos of the amphibian Xenopus laevis.

Gli2 is required for the induction and migration of Xenopus laevis neural crest.

The neural crest (NC) is a multipotent migratory embryonic population that is formed during late gastrulation and gives rise to a wide array of derivatives, including cells from the peripheral nervous system (PNS), the craniofacial bones and cartilages, peripheral glial cells, and melanocyte cells, among others. In this work we analyzed the role of the Hedgehog signaling pathway effector gli2 in Xenopus NC. We provide evidence that the gli2 gene is expressed in the prospective, premigratory and migratory NC.

Neurocristopathies: New insights 150 years after the neural crest discovery.

The neural crest (NC) is a transient, multipotent and migratory cell population that generates an astonishingly diverse array of cell types during vertebrate development. These cells, which originate from the ectoderm in a region lateral to the neural plate in the neural fold, give rise to neurons, glia, melanocytes, chondrocytes, smooth muscle cells, odontoblasts and neuroendocrine cells, among others. Neurocristopathies (NCP) are a class of pathologies occurring in vertebrates, especially in humans that result from the abnormal specification, migration, differentiation or death of neural crest cells during embryonic development.

Periodic venting of MABR lumen allows high removal rates and high gas-transfer efficiencies.

The membrane-aerated biofilm reactor (MABR) is a novel treatment technology that employs gas-supplying membranes to deliver oxygen directly to a biofilm growing on the membrane surface. When operated with closed-end membranes, the MABR provides 100-percent oxygen transfer efficiencies (OTE), resulting in significant energy savings. However, closed-end MABRs are more sensitive to back-diffusion of inert gases, such as nitrogen.

Associated anomalies and clinical outcome in children with ectopic kidney.

Urological anomalies can be seen in children with renal ectopia (RE) and can result in renal impairment. Therefore, we evaluated associated anomalies and renal outcome in our patients with RE. Sixty-eight children who were diagnosed with RE between January 2009-May 2014 were retrospectively studied.

Trunk neural crest cells: formation, migration and beyond.

Neural crest cells (NCCs) are a multipotent, migratory cell population that generates an astonishingly diverse array of cell types during vertebrate development. The trunk neural crest has long been considered of particular significance. First, it has been held that the trunk neural crest has a morphogenetic role, acting to coordinate the development of the peripheral nervous system, secretory cells of the endocrine system and pigment cells of the skin.