An efficient in vitro-inoculation method for Tomato yellow leaf curl virus.

Background: Tomato yellow leaf curl virus (TYLCV) is a member of the family Geminiviridae, genus Begomovirus. To test the infectivity of TYLCV in tomato plants, an improved protocol for inoculation of in vitro-cultured tomato plants was developed.

Results: A TYLCV isolate was cloned, sequenced and used to construct a 1.