Effect of antimicrobial photodynamic therapy using rose bengal and blue light-emitting diode on : Influence of oxygen during treatment.

A combination of rose bengal (RB) and blue LED (BL) has emerged as a new technical modality for antimicrobial photodynamic therapy (a-PDT). The purpose of this study was to clarify the influence of oxygen on the antimicrobial effect of RB + BL treatment on cells were treated with RB, BL (450-470 nm; 1 W/cm, 5 s), or RB + BL under anaerobic/aerobic conditions. Cells were incubated anaerobically, and the cell density (OD) was measured after 6-48 h.