Publications by authors named "Ayane Sagawa"
Messenger RNA (mRNA)-based therapies are a novel class of therapeutics used in vaccination and protein replacement therapies for monogenic diseases. Previously, we developed a modified ethanol injection (MEI) method for small interfering RNA (siRNA) transfection, in which cationic liposome/siRNA complexes (siRNA lipoplexes) were prepared by mixing a lipid-ethanol solution with a siRNA solution. In this study, we applied the MEI method to prepare mRNA lipoplexes and evaluated the in vitro and in vivo protein expression efficiencies.
View Article and Find Full Text PDFArticle Synopsis
- * Researchers tested three cationic lipids (DOTAP, DDAB, and TC-1-12) with various phosphatidylcholine and phosphatidylethanolamine structures to find the best formulations for gene delivery.
- * The study concluded that specific lipid combinations led to effective gene knockdown in human cancer cells and in mouse lungs, highlighting that the type of cationic lipid significantly affects transfection efficiency.