Publications by authors named "Ayanda Manqele"

Article Synopsis
  • The study focused on characterizing a common bacterial pathogen found in beef and its products using whole-genome sequencing (WGS) on 24 isolates.
  • The most prevalent sequence type was identified as ST204, along with several others, and many isolates carried genes related to virulence and antibiotic resistance.
  • Key findings emphasized the potential risk this bacterium poses to public health due to its presence in food environments, highlighting both its virulence factors and environmental resistance traits.
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Abstract: A cross-sectional study was conducted to determine the prevalence of and factors associated with Shiga toxin-producing Escherichia coli (STEC) in raw beef and ready-to-eat (RTE) beef products sold in 31 retail outlets in Pretoria, South Africa, and nearby areas. A total of 463 beef and RTE samples were screened for four STEC virulence genes (stx1, stx2, eaeA, and hlyA) and seven O-serogroups (O113, O157, O26, O91, O145, O111, and O103) with a multiplex PCR assay. The total aerobic plate count (TAPC) per gram was also determined.

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