Publications by authors named "Ayana Tabo"
Single-molecule diffusivity quantification in egg extracts elucidates physicochemical properties of the cytoplasm.
Proc Natl Acad Sci U S A
December 2024
Article Synopsis
- The study investigated the unique molecular environment of living cells by analyzing the diffusion of various proteins in cytoplasmic extracts from eggs using a technique called single-molecule displacement/diffusivity mapping (SMM).
- It was found that negatively charged proteins diffused about 50% slower, while positively charged proteins showed a much greater reduction in diffusion by 80-90%, suggesting strong electrostatic interactions in the predominantly negatively charged cytoplasm.
- The research also identified that the presence of RNA and cytoskeletal components, like actin, significantly affects protein diffusion and viscosity, indicating the complex interactions at play within the crowded cellular environment.
Unlabelled: The living cell creates a unique internal molecular environment that is challenging to characterize. By combining single-molecule displacement/diffusivity mapping (SM M) with physiologically active extracts prepared from eggs, we sought to elucidate molecular properties of the cytoplasm. Quantification of the diffusion coefficients of 15 diverse proteins in extract showed that, compared to in water, negatively charged proteins diffused ∼50% slower, while diffusion of positively charged proteins was reduced by ∼80-90%.
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