Absence of Gene Is Concordant with Femaleness in Cichlids Harboring the LG1 Sex-Determination System.

has been used as a reference genome for studies of tilapia sex determination (SD) revealing segregating genetic loci on linkage groups (LGs) 1, 3, and 23. The master key regulator genes (MKR) underlying the SD regions on LGs 3 and 23 have been already found. To identify the MKR in fish that segregate for the LG1 XX/XY SD-system, we applied short variant discovery within the sequence reads of the genomic libraries of the hybrid stock, and , which were aligned to a 3-Mbp-region of the genome.

Gene Variant of () Is Concordant with Female Determination in Cichlids.

fishes exhibit variability of sex-determination (SD) genes whose characterization contributes to understanding of the sex differentiation network, and to effective tilapia farming, which requires all-male culture. However, () is the only master-key regulator (MKR) of SD that has been mapped (XY/XX SD-system on LG23). In (), LG3 controls a WZ/ZZ SD-system that has recently been delimited to 9.

Quantitative trait loci on LGs 9 and 14 affect the reproductive interaction between two Oreochromis species, O. niloticus and O. aureus.

Effective farming of tilapia requires all-male culture, characterized by uniformity and high growth rate. Males of O. aureus (Oa) and females of O.