Haematological parameters as predictors of severe dengue: a study from northern districts of West Bengal.

Background Objectives: A hike in dengue cases was recorded in last two years, resulting from both single and multiple-serotypes of dengue virus (DENV) and secondary infections, culminating in significant hospitalizations and deaths in India. This study focuses on evaluating symptomatic and haematological parameters in acute dengue patients of the northern part of West Bengal to predict disease severity early on and also to analyze the correlation between circulating DENV serotypes with severity.

Methods: Dengue patients (N=540) diagnosed as NS1 positives were categorized into 13.

Inhibition of NF-κB-Mediated Proinflammatory Transcription by Ru(II) Complexes of Anti-Angiogenic Ligands in Triple-Negative Breast Cancer.

Nuclear factor kappa beta (NF-κB) plays a pivotal role in breast cancer, particularly triple-negative breast cancer, by promoting inflammation, proliferation, epithelial-mesenchymal transition, metastasis, and drug resistance. Upregulation of NF-κB boosts vascular endothelial growth factor (VEGF) expression, assisting angiogenesis. The Ru(II) complexes of methyl- and dimethylpyrazolyl-benzimidazole , donors inhibit phosphorylation of ser536 in p65 and translocation of the NF-κB heterodimer (p50/p65) to the nucleus, disabling transcription to upregulate inflammatory signaling.

Cytotoxic Ruthenium(II) Complexes of Pyrazolylbenzimidazole Ligands That Inhibit VEGFR2 Phosphorylation.

Eight new ruthenium(II) complexes of ,-chelating pyrazolylbenzimidazole ligands of the general formula [Ru(-cym)(L)X] [where the ligand L is 2-(1-pyrazol-1-yl)-1-benzo[]imidazole () substituted at the 4 position of the pyrazole ring by Cl (), Br (), or I () and X = Cl and I] were synthesized and characterized using various analytical techniques. Complexes and were also characterized by single-crystal X-ray crystallography, and they crystallized as a monoclinic crystal system in space groups 2/ and 2/, respectively. The complexes display good solution stability at physiological pH 7.

Synthesis, Structure and Cytotoxicity of N,N and N,O-Coordinated Ru Complexes of 3-Aminobenzoate Schiff Bases against Triple-negative Breast Cancer.

Half-sandwich Ru complexes, [(YZ)Ru (η -arene)(X)]+, (YZ=chelating bidentate ligand, X=halide), with N,N and N,O coordination (1-9) show significant antiproliferative activity against the metastatic triple-negative breast carcinoma (MDA-MB-231). 3-aminobenzoic acid or its methyl ester is used in all the ligands while varying the aldehyde for N,N and N,O coordination. In the N,N coordinated complex the coordinated halide(X) is varied for enhancing stability in solution (X=Cl, I).

Label-free detection of thalassemia and other ROS impairing diseases.

Pathogenesis of different diseases showed that some of them, especially thalassemia (T) and rheumatoid arthritis (RA) have an implicit association with oxidative stress and altered levels of reactive oxygen species (ROS). Introducing ROS level and the balance between ROS and antioxidants as essential metrics, an attempt was made to classify T and RA from normal individuals (treated as controls)(C) using synchronous fluorescence spectroscopy (SFS) and Raman line intensity of water. This non-invasive and label-free approach was backed up by a categorization algorithm that helped in the prediction of disease types from serum samples.

Non-synonymous amino acid alterations in PfEBA-175 modulate the merozoite ligand's ability to interact with host's Glycophorin A receptor.

The pathological outcome of malaria due to Plasmodium falciparum infection depends largely on erythrocyte invasion by blood-stage merozoites which employ a cascade of interactions occurring between parasite ligands and RBC receptors. In a previous study exploring the genetic diversity of region-II of PfEBA-175, a ligand that plays a crucial part in parasite's RBC entry through Glycophorin A (GPA) receptor, we demonstrated that F2 domain of region-II underwent positive selection in Indian P. falciparum population through the accumulation of non-synonymous polymorphisms.

Intracellular iron overload leading to DNA damage of lymphocytes and immune dysfunction in thalassemia major patients.

Objectives: To investigate the cause and effects of intracellular iron overload in lymphocytes of thalassemia major patients.

Methods: Sixty-six thalassemia major patients having iron overload and 10 age-matched controls were chosen for the study. Blood sample was collected, and serum ferritin, oxidative stress; lymphocyte DNA damage were examined, and infective episodes were also counted.

A Tiered Approach to Evaluating Salinity Sources in Water at Oil and Gas Production Sites.

A suspected increase in the salinity of fresh water resources can trigger a site investigation to identify the source(s) of salinity and the extent of any impacts. These investigations can be complicated by the presence of naturally elevated total dissolved solids or chlorides concentrations, multiple potential sources of salinity, and incomplete data and information on both naturally occurring conditions and the characteristics of potential sources. As a result, data evaluation techniques that are effective at one site may not be effective at another.