Publications by authors named "Ayame Ogawa"
Diversity in the granulocyte repertoire, including neutrophils, basophils, and eosinophils, has been reported in vertebrate species. Medaka fish (Oryzias latipes) have only neutrophils; however, the storage pool of granulopoiesis tissues and the molecular mechanism of granulopoiesis in medaka fish have not been explored. Granulocyte colony-stimulating factor (G-CSF) is a cytokine responsible for neutrophil differentiation, survival, and proliferation.
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- The study investigates the localization and development of myeloid cells in transgenic frogs, specifically focusing on myeloperoxidase-positive cells in the liver cortex during late tadpole stages.
- Findings show that myelopoiesis in the liver cortex starts at stage 50 and peaks by stage 56, with transplantation experiments revealing these cells originated from the dorso-lateral plate in the neurula embryo.
- Analysis of cells isolated from the liver indicates that myeloid cells dominate the blood cell population in larval liver, and following metamorphosis, there is an expansion of both myeloid and erythroid colonies, confirming the liver cortex as a key site for granulopoiesis.
Article Synopsis
- DNA polymerase epsilon (Pol ε) is crucial for DNA replication and its genetic defects are linked to immunodeficiency syndromes; however, its impact on blood cell formation (haematopoiesis) is unclear.
- Researchers discovered specific genetic variants in siblings with severe congenital anemia, which demonstrated atypical symptoms compared to typical cases like Diamond-Blackfan anemia.
- Further studies using patient-derived stem cells and medaka fish models revealed reduced function of Pol ε, leading to blood cell deficiencies and enhancing our understanding of its role in haematopoiesis.
Article Synopsis
- * Using transgenic Xenopus laevis, we found that GFP (green fluorescent protein) expression indicated the presence of myeloid cells in tadpole tail fin macrophages and liver areas.
- * Analysis of blood leukocytes showed that GFP-positive cells were primarily neutrophils, and macrophages from the peritoneal cavity were also GFP-positive, highlighting the utility of this transgenic model for studying myeloid cell functions in embryos.
Background: A globus sensation is one of the most common complaints in otolaryngological practice. Patients with no associated abnormalities detected during the usual examinations performed in ENT clinics, are being diagnosed with globus sensation. Cervical ultrasonography is usually not performed in ENT clinics; however, it is useful in screening diseases of the subcutaneous tissue/organs, whose detection is not possible with the routine ENT examinations.
View Article and Find Full Text PDFGastrointestinal stromal tumor (GIST) is the most common submucosal tumor of the stomach. GISTs are often detected by esophagogastroduodenal endoscopy. We have previously reported on endoscopically invisible medium-sized exophytic type GISTs.
View Article and Find Full Text PDFBackground: This study aims to elucidate the effect of isolated cerebellar lesions sparing the brainstem on the auditory brainstem responses in children.
Methods: We enrolled 10 children (aged 1-16 years) with cerebellar lesions on neuroimaging but lacking clinical brainstem involvement signs and with normal brainstem volumes on magnetic resonance imaging.
Results: The interpeak latency of waves I and V was normal in 9 patients and was marginally prolonged in 1 patient.