Genomics and Virulence of Kpnu95 ST1412 Harboring a Novel Incf Plasmid Encoding ctx-M-15 and Causing Community Urinary Tract Infection.

The emergence of extended-spectrum β-lactamase (ESBL)-producing multidrug resistant causing community urinary tract infections (CA-UTI) in healthy women undermines effective treatment and poses a public health concern. We performed a comprehensive genomic analysis (Illumina and MinION) and virulence studies using nematodes to evaluate KpnU95, a -producing CA-UTI strain. Whole genome sequencing identified KpnU95 as sequence type 1412 and revealed the chromosomal and plasmid-encoding resistome, virulome and persistence features.

Assembly of cationic and amphiphilic β-sheet FKF tripeptide confers antibacterial activity.

The race drawn against bacteria facing the evolution of antimicrobial resistance fuels research for new drugs and therapeutic strategies. FKF, a tripeptide that is cationic and amphiphilic was examined in light of its potential antimicrobial activity. Acid titration of purified peptide solution, 6% w/v (136 mM), yielded a hydrogel at pH~ 4.