The accuracy of quantifying the degree of hard tissue calcification using an electron probe micro analyzer, micro-focus X-ray computed tomography, and tissue sectioning methods.

Objectives: Micro-focus X-ray computed tomography (μCT) helps evaluate specimens without destroying it. However, its accuracy of quantifying bone mineral density remains to be fully elucidated. We aimed to verify the accuracy of calcification assessed by μCT, by comparing the images of identical specimens obtained via different methods such as μCT and electron probe micro analyzer (EPMA) analyses.

Distinct differences between cultured podocytes and parietal epithelial cells of the Bowman's capsule.

Phenotypic changes in culture hamper the identification and characterization of cultured podocytes and parietal epithelial cells of the Bowman's capsule (PECs). We have recently established culture conditions that restore podocytes to their differentiated phenotypes. We compared podocytes and PECs cultured under the same conditions to determine the unique characteristics of the two cell types.