Comparative Evaluation of Open-Source Bioinformatics Pipelines for Full-Length Viral Genome Assembly.

The increasingly widespread application of next-generation sequencing (NGS) in clinical diagnostics and epidemiological research has generated a demand for robust, fast, automated, and user-friendly bioinformatics workflows. To guide the choice of tools for the assembly of full-length viral genomes from NGS datasets, we assessed the performance and applicability of four open-source bioinformatics pipelines (shiver-for which we created a user-friendly Dockerized version, referred to as dshiver; SmaltAlign; viral-ngs; and V-pipe) using both simulated and real-world HIV-1 paired-end short-read datasets and default settings. All four pipelines produced consensus genome assemblies with high quality metrics (genome fraction recovery, mismatch and indel rates, variant calling F1 scores) when the reference sequence used for assembly had high similarity to the analyzed sample.

The impact of a walking program on self-management, anxiety, stress, depression, quality of life, and seizure frequency in patients with epilepsy: A mixed methods approach using the COM-B behaviour change model.

Objective: This study aimed to investigate the effect of a walking program based on the COM-B Behavior Change Model on self-management, anxiety, stress, depression, quality of life, and seizure frequency in individuals with epilepsy.

Methods: A prospective, parallel-group controlled experimental design and mixed methods were used. A total of 78 individuals with epilepsy were included in the study and randomly assigned to the intervention (n = 38) and control (n = 40) groups.

Violence tendency and internet addiction in adolescents.

Objective: The aim of this study was to determine the relationship between internet addiction and violence tendency in adolescent students and the factors affecting violence tendency.

Methods: The research was conducted on 2,648 middle school students in Turkey. A socio-demographic form, the Young Internet Addiction Scale, and the Violence Tendency Scale were used to collect data.

The therapeutic potential and molecular mechanism of , and against melanoma cells.

The purpose of this study is to investigate the potential anticarcinogenic effects of three phytochemicals, namely (AP), (GT), and (PC), on melanoma cells (A2058 cell line). Additionally, the study aims to explore the synergistic activities of these phytochemicals with Dacarbazine, a chemotherapy drug. To understand the molecular mechanism involved in apoptosis induction in the A-2058 cell line, it was used AO/EB staining for apoptosis detection and cell cycle analysis, monitored through flow cytometry.

The mediating role of anxiety in the relationship between misophonia and quality of life: findings from the validated Turkish version of MisoQuest.

Introduction: Misophonia is a disorder characterized by decreased tolerance to certain sounds or their associated stimuli, and many measurement tools have been developed for its diagnosis and evaluation. The aims of the current study were to develop the Turkish version of MisoQuest, a fully validated misophonia questionnaire, to evaluate the relationships between misophonia, anxiety, and quality of life, and to examine the mediating role of anxiety in the relationship between misophonia and quality of life.

Methods: The reliability of the Turkish version of MisoQuest was conducted using data from 548 participants (Mean age = 28.

Is poor oral health a risk factor for idiopathic granulomatous mastitis?

Idiopathic granulomatous mastitis (IGM) is a rare inflammatory breast disease that can be clinically and radiologically mistaken for carcinoma. Although its etiology remains uncertain, potential associations with pregnancy, lactation, hormonal imbalances, autoimmunity, smoking, and various microorganisms have been suggested. This study aimed to evaluate the relationship between IGM and oral health.

Examination of internalized stigma, quality of life, and happiness in patients with schizophrenia.

Background: Happiness in patients with schizophrenia is a neglected concept that needs to be developed. Determining the relationship between internalized stigma, quality of life, and happiness, which is important for the prognosis of schizophrenia, will reveal why it is necessary to focus on activities that will increase happiness in patients with schizophrenia.

Aim: This study aimed to compare the levels of internalized stigma, quality of life, and happiness in patients with schizophrenia.

Validity and reliability study of the Turkish Personal Report of Intercultural Communication Apprehension Scale: a revised scale.

Background: Intercultural communication is one of the most important competencies of nurses that enhances the health-care process and increases the quality of care in multicultural environments.

Aims: This study was conducted to revise the Turkish Personal Report of Intercultural Communication Apprehension Scale.

Methods: This methodological study was conducted between November and December 2021 with 321 female and 96 male university students (mean age 20.

A new surgical path planning framework for neurosurgery.

Background: Despite using a variety of path-finding algorithms that use tracts, the most significant advancement in this study is considering the values of all brain areas by doing atlas-based segmentation for a more precise search. Our motivation comes from the literature's shortcomings in designing and implementing path-planning methods. Since planning paths with curvatures is a complex problem that requires considering many surgical and physiological constraints, most path-planning strategies focus on straight paths.

Evaluation of the relationship between food addiction and depression, anxiety, and stress in university students: A cross-sectional survey.

Purpose: This study was conducted to determine the relationship between food addiction (FA) and depression, anxiety, and stress (DAS) in university students.

Design And Methods: The research was conducted with 362 university students who met the study criteria and volunteered to participate in the study. The study data were collected using a personal information form, the modified Yale food addiction scale 2.

Hearing Loss Related to Gene Mutations in Distal Renal Tubular Acidosis.

Introduction: Distal renal tubular acidosis (dRTA) is a disease that may develop either primarily or secondarily, resulting from urinary acidification defects in distal tubules. Hearing loss may accompany primary forms of dRTA. This study aims to determine the characteristics of hearing loss due to different gene mutations in patients with dRTA.

Synthesis of new 1,2,3-triazolo-nucleoside analogues with 2-propargylamino pyrimidines click reactions.

In this study, it was reported that twelve nucleoside analogues were synthesized by click reactions. The reactions were carried out between the azide derivatives of D-glucopyranose, D-galactopyranose, D-ribofuranose and propargylamino pyrimidine derivatives ( and ) that are synthesized a different route for the first time. In the first step, -propargyl guanidine was obtained with the reaction of 1-pyrazole-1-carboxamidine hydrochloride and propargyl amine, then condensation of -propargyl guanidine and -diketone ( and ) resulted in 2-propargylamino pyrimidines ( and ) for the first time in good yields (85%).

Investigation of possible associations between tryptophan/kynurenine status and FOXP3 expression in colorectal cancer.

Tryptophan metabolism in the tumor microenvironment exerts immunosuppressive effects by affecting the anti-tumor functions of immune cells. The immunosuppressive roles of tryptophan and tryptophan metabolites and their effects on the FOXP3 gene, highly expressed in regulatory T cells (Tregs), are remarkable. Our study aimed to investigate the relation between tryptophan metabolism and the transcription factor FOXP3 gene in colorectal cancer (CRC).

New chalcone derivatives as effective against SARS-CoV-2 agent.

Aims: Flavonoids and related compounds, such as quercetin-based antiviral drug Gene-Eden-VIR/Novirin, inhibit the protease of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The alkylated chalcones isolated from Angelica keiskei inhibit SARS-CoV proteases. In this study, we aimed to compare the anti-SARS CoV-2 activities of both newly synthesized chalcone derivatives and these two drugs.

Genotyping, drug resistance and virulence factors of Candida species isolated from patients using long-term inhaled steroids.

Aims: In this study, it was aimed to determine the isolation frequency and species distribution of Candida species isolated from asthmatic patients using long-term inhaled steroids. It was also aimed to determine the drug resistance patterns and the frequency of erg11, HWP1, ALS1, INT1, SAP1 PLB1 genes in isolates.

Methods: Genotyping of Candida strains isolated from patients and healthy control group was performed by PCR-RFLP method.

Photostability of l-tryptophan in aqueous solution: Effect of atmosphere and antioxidants addition.

l-Tryptophan (l-Trp) is an amino acid important in nutrition, and mainly provided by food supplements. However, it is known to be unstable under light irradiation, which is an issue for the nutrition and feed industry. In the present study, the photostability of l-Trp was studied in acidic aqueous solutions under air and under an inert atmosphere, N.

First determination of some phenolic compounds and antimicrobial activities of Geranium ibericum subsp. jubatum: A plant endemic to Turkey.

This paper includes the results of the first study about the phenolic characteristics and antimicrobial analyses of subsp. species found in Turkey In the present work, the phenolic contents of different parts of the (flower, root, leaf) were determined with high-performance liquid chromatography (HPLC)-DAD (diode-array detector) and liquid chromatography (LC)-MS/MS (mass spectrometry). The following phenolic compounds were investigated: catechin, protocatechuic acid, gallic acid, ellagic acid, chlorogenic acid, 4-hydroxybenzaldehyde, -coumaric acid, rutin, naringenin, kaempferol Based on the results obtained, the roots and flowers of the plant are found to be very rich in ellagic acid (3473.

Reference intervals for serum amyloid A, haptoglobin, ceruloplasmin, and fibrinogen in apparently healthy neonatal lambs.

Background: Acute-phase proteins (APPs) are considered biomarkers for diagnoses, prognoses, and treatment and health status monitoring. Reference intervals (RIs) are an integral part of diagnostic laboratory testing and clinical decision-making. The RIs for neonatal lambs are lacking.

Dying in the Intensive Care Unit (ICU): A Retrospective Descriptive Analysis of Deaths in the ICU in a Communal Tertiary Hospital in Germany.

Background: Modern intensive care methods led to an increased survival of critically ill patients over the last decades. But an unreflected application of modern intensive care measures might lead to prolonged treatment for incurable diseases, and an inadaequate or too aggressive therapy can prolong the dying process of patients. In this study, we analysed end-of-life decisions regarding withholding and withdrawal of intensive care measures in a German intensive care unit (ICU) of a communal tertiary hospital.

Ascorbic Acid Derivatives as Potential Substitutes for Ascorbic Acid To Reduce Color Degradation of Drinks Containing Ascorbic Acid and Anthocyanins from Natural Extracts.

Ascorbic acid is widely used in the food industry as a source of vitamin C or as antioxidant. However, it degrades quickly in beverages at acidic pH and can accelerate the degradation of anthocyanins, natural dyes used in beverages, leading to a loss of color. In this work, we investigated the possibility to replace ascorbic acid by ascorbic acid derivatives to prevent its degradation effect on anthocyanins from natural extracts (black carrot, grape juice, and purple sweet potato).

Assessment of the relationship between coronary flow rates and myocardial perfusion abnormality in patients with nonobstructive coronary artery disease: an observational study in cardiac syndrome X and coronary slow flow.

Objectives: In this study, we evaluated and compared the level of myocardial ischaemia caused by cardiac syndrome X (CSX) and coronary slow flow (CSF) with single photon emission computed tomography myocardial perfusion imaging (SPECT-MPI), and determined if changes in the level of myocardial ischaemia exist in CSF and CSX cases according to thrombolysis in myocardial infarction frame count (TFC).

Materials And Methods: The study population consisted of 66 patients with CSF and 78 angiographically normal patients (36 of them with CSX and 42 of them healthy controls). The coronary flow rates of all patients were documented using TFC.