Bilateral cavitary multidrug- or extensively drug-resistant tuberculosis: role of surgery.

Objectives: Cavitary disease and bilateral lesions are among the risk factors for poor outcome of pulmonary tuberculosis (TB). Our aim was to explore the value and limits of surgery in patients with advanced TB.

Methods: A retrospective study of 57 consecutive patients who underwent thoracic surgery for culture-positive bilateral cavitary pulmonary TB was performed.

[Comparative analysis of the informative value of 18F-fluorodeoxyglucose and 11C-methionine positron emission tomography in the differential diagnosis of solitary lung masses].

Objective: To study the possibilities of enhancing the efficiency of differential diagnosis of solitary lung masses by optimizing the quantitative analysis of positron emission tomography (PET) data when two radiopharmaceuticals (RP) (18F-fluorodeoxyglucose (18F-FDG) and 11C-methionine) are used alone and in combination.

Material And Methods: A comprehensive examination involving 18F-FDG and 11C-methionine PET was made in 116 patients with solitary lung masses of various genesis. A final diagnosis in the examinees was established from the results of postoperative material morphological analysis and/or laboratory tests and those of X-ray follow-up.

[Influence of ionizing radiation on enzymatic activity and state of nucleus-nucleolar apparatus in rat hepatocytes].

The effects of a single exposure of rats to the whole-body roentgen irradiation at the doses of 3.5 Gy and 4.5 Gy on the activity of creatine kinase, purine nucleoside phosphorylase, alanine aminotransferase, aspartate aminotransferase, as well as on the state of the nuclear-nucleolar apparatus in rat hepatocytes on the 6th and 13th days after radiation exposure have been studied.

[Multidrug resistance P-glycoprotein inhibits the antiapoptotic action of mitochondria-targeted antioxidant SkQR1].

Mitochondria-targeted antioxidants of the SkQR1 family, being accumulated in energized mitochondria, protect cells from oxidative stress by increasing the level of reduced glutathione and decreasing the cell-damaging effect induced by hydrogen peroxide. Using various human transformed cell lines and SkQR1 (a fluorescent member of the SkQ family), we show that SkQRI is ejected from chemotherapy-resistant cells by P-glycoprotein - one of the main transport proteins determining multidrug resistance typical for many neoplastic cells. It is also shown that SkQR1 ejection is neutralized by P-glycoprotein inhibitors (verapamil and pluronic L61).

[The reaction of the neuroblastoma cells in the culture on the influence of tretionine and neurotoxine].

Effect of the tretionine (retinoid) and aluminum chloride (neurotoxin) on the growth and differentiation of neuroblastoma cells in culture after their introduction into the medium separately and in combination was studied. The introduction of these substances creates a new information field in the medium, which becomes apparent by the reactions of neuroblastoma found on the populational and cellular levels of its organization. The presence of tretionine stimulates proliferation and induces differentiation of the cells into astrocytes.

[Pathology of lymphoid tissue cells infected by African swine fever virus in vitro].

The authors studied the pathology of bone marrow (BM) lymphoid cell from pigs infected by African swine fever virus (ASFV) in vitro. Monocytes were shown to be primarily afflicted in unstimulated BM culture. These cells disappeared completely 72 hours after infection.

[Multidrug resistance p-glycoprotein inhibits the antiapoptotic action of mitochondria-targeted antioxidant SkQR1].

Mitochondria-targeted antioxidants of the SkQRI family, being accumulated in energized mitochondria, protect cells from oxidative stress by increasing the level of reduced glutathione and decreasing the cell-damaging effect induced by hydrogen peroxide. Using various human transformed cell lines and SkQR1 (a fluorescent member of the SkQ family), we show that SkQR1 is ejected from chemotherapy-resistant cells by P-glycoprotein--one of the main transport proteins determining multidrug resistance typical for many neoplastic cells. It is also shown that SkQR1 ejection is neutralized by P-glycoprotein inhibitors (verapamil and pluronic L61).

[Immunological changes in patients with pulmonary tuberculosis during glutoxim therapy].

The paper deals with the effect of glutoxim included into a preoperative preparation regimen on immunological parameters in patients with fibrocavernous pulmonary tuberculosis. On admission, all the patients had inadequate cellular immunity and activated humoral immunity. After termination of a course of glutoxim therapy, there was an increase in the baseline low values of lymphocytic proliferative activity, in the count of mature T lymphocytes, and in the production of IL-2 induced by phytohemagglutinin.

[Significance of mycobacterial drug resistance in pulmonary tuberculosis surgery].

The outcomes of surgical treatment of 546 patients with pulmonary tuberculosis were analyzed in relation to the bacteriological characteristics. Seeding detected sputum bacterial isolation in 52.4% of cases.

[New technology in craniofacial human identification].

Results of development and prospects of using new computer technologies of craniofacial personality identification based on three-dimensional simulation of identification objects are demonstrated.

[Stimulating effect of sodium ions on Escherichia coli growth in the presence of protonophore uncoupler].

The increase of Na+ concentration from the submillimolar level to 50 mM stimulates growth of the Escherichia coli cells in the presence of the protonophorous uncoupler CCCP. The half-maximal effects is observed at 1 mM Na+. The decrease of inorganic phosphate concentration from tens of millimoles to 0.

[Immunocytophotometric and cytogenetic analyses of T-lymphoblastoid cell lines in experimental HIV Infection].

The accumulation of viral proteins p24 and gp41 in MT-4 cells was shown to occur asynchronously. No increase in the number of polydiploid cells, no polyploid mitoses or chromosome defects were found indicating the antiproliferative effect of HIV on MT-4 cells. A cyclic pattern of the infection course was observed in infected Jurkat-tat cells.

[A surgical method for the treatment of traumatic low-solubility hyphema].

Presents experimental and clinical data on the operation of 'angular drainage' of the anterior chamber in traumatic poorly soluble hyphemas. The operation consists in conduction of a supramide thread through performed orifices in the cornea and formation of an angular drainage for blood evacuation from the anterior chamber. High efficacy and safety of the suggested method for treatment of recurrent complicated hyphemas is proved.

[Effect of dye quality and the method of preparing a Schiff reagent on the staining intensity and DNA-fuchsin absorption spectra in performing the Feulgen reaction].

Dependence of the staining and absorption spectra of DNA-fuchsin on the dye quality and modes of preparation of the Schiff-reagent was studied. It is shown that many dyes supplied by different producers do not suit the purposes of quantitative cytochemical investigations. Before use the absorption spectra of the DNA-fuchsin must be taken using the standard object.

[Role of the supraoptic nucleus in the estrus regulation in rats in local hypothermia].

An extraordinary role of hypothalamic supraoptical nuclei in the ovulation process and luteinizing hormone formation was found out. Morphological studies of the rat ovaries with the affected supraoptical nuclei have shown a sharp inhibition of their function, manifesting in the decreased follicular number of sizes, atresia of primordial follicles, the inhibition of the ovarian ovulatory capacity and luteinizing hormone formation. The ovarian function remained inhibited, despite increased neurosecretory activity of the paraventricular nucleus cells in rats with the affected hypothalamic supraoptic nuclei during hypothermia.

[Effect of the hydrolyzing solution temperature, acid concentration and the duration of the hydrolysis on the intensity of the Feulgen reaction].

The quantity of DNA-fuchsin in the cock monocytes and erythrocytes has been measured by scanning cytophotometric methods and computer analysis. Hydrolysis was carried out using 1 N HCl (60 degrees C, 4-30 min) and 5 N HCl (37 degrees C, 4-36 min, and 22 degrees C, 10-150 min). The elevation of the temperature from 22 degrees to 37 degrees resulted in a 5-fold reducing of the time required for achieving the maximum of the hydrolysis curve, although the DNA content at the maximum point decreased by 7-9%.

[Intensity of the Feulgen reaction depending on the method and duration of fixation].

A comparative cytophotometric investigation of DNA-fuchsine content in mature erythrocytes of chicks was carried out using various methods and duration of fixation: 96 degrees and 100% ethanol, Carnoy's fixative, and mixtures of 40% formalin--96% ethanol--glacial acetic acid /GAA/(9 : 3 : 1), 100% methanol--4% formalin--GAA (17 : 2 : 1), and 96% ethanol--GAA (3 : 1)+formalin (final concentration 2%). The duration of fixation varied from 15 to 150 minutes, with 15 minutes intervals. It has been shown that the quantity of DNA--fuchsine (under equal conditions of the Feulgen reaction) depends greatly on the method and duration of fixation.

[Certain problems in developing the Automated Control System for the "Sanitary-Epidemiologic Service" of the Uzbek SSR].

The main difficulties of using the subsystem "Records, Accounts and Analysis of Morbidity in Infectious Diseases", developed by the Republican Information and Computing Center of the Ministry of Health of the Byelorussian SSR for integration into the automated control system, under conditions of the Uzbek SSR lied in the fact that the number of the coupons of Recond Forms 171-BM for computers, several times exceeding that used in the Byelourssian SSR, had to be previously handled manually and in a short time.