Prediction of graft versus host disease by frequency analysis of cytotoxic T cells after unrelated donor bone marrow transplantation.

HLA "matched" unrelated donor bone marrow transplants are associated with an increased incidence and severity of graft-versus-host disease in comparison with HLA-identical sibling transplants. This is presumably due to HLA and non-HLA histocompatibility differences between donor and recipient. Using a limiting dilution assay, we have previously demonstrated a relationship between cytotoxic T lymphocyte precursor frequency and HLA disparity.

[The action of azepoxole on central alpha 2-adrenoreceptors].

The effect of adrenomimetics on the central alpha 2-adrenoreceptors responsible for mydriasis was studied. It was found that alpha 2-adrenomimetic azepoxole (BHT-933) exerts no effect on the central alpha 2-adrenoreceptors providing mydriasis.

[The rat vas deferens as a test object for assessing the action of chemical compounds].

The vas deferens is widely used for studies of the action of chemical compounds on adrenergic processes. The recent data indicate that the response of the vas deferens to transmural electric stimulation can be blocked by the substances possessing different properties and that to have a correct assessment of the action of new chemical compounds, of great importance is both the animal species and the characteristics of the strips used (epididymal and prostatic) and electric impulses. The epididymal strip of the rat vas deferens--transmural stimulation by serial electric impulses is a convenient model for screening new chemical compounds.

[Effect of odiphalin and difril on catecholamine concentration in adrenergic nerve fibers of different rat organs].

The histochemical method of Axelsson coupled with photometry was used to study variations in the content of biogenic amines in different rat organs (myocardium, vas deferens, mesenteric vessels, iris, atrial and ventricular heart valve) under the effect of odiphalin and difril. The results obtained indicate that the organs with direct, indirect and mixed innervation differ in the pattern of response to the pharmacological agents administered. Venous vessels proved to be the most sensitive to the action of odiphalin and difril.

[Development of the adrenergic innervation of the atrioventricular valves of certain vertebrates].

Adrenergic innervation of the atrioventricular valves in the carp Cyprinus carpo, frog Rana temporaria, tortoise Emys orbicularis, hens and albino rats has been investigated by histochemical glyoxylic technique. It was shown that the adrenergic innervation of cardiac valves becomes more intense and more complex during progressive evolution of cardio-vascular activity. Adrenergic nerves realize the connection between annullus fibrosus, valve, chordal tendineal and papillary muscles.

[Effect of adrenalectomy and chemical sympathectomy on the catecholamines content in the central nervous system in hemorrhagic stress].

Content of adrenaline, noradrenaline, dopamine and normetanephrine was estimated by means of trihydroxyindole method in samples of brain and its hypothalamic part from intact, adrenal- and sympathectomized rats within 15 min, 24 and 72 hrs after hemorrhagic stress (3% blood of body weight within 20 min). Stable increase in dopamine content was observed in brain at all the periods studied. Alterations in the noradrenaline turnover in central nervous system were related to the state of peripheral sympathoadrenal system at early posthemorrhagic steps; the phenomenon was not found at the later periods after stress.

[New model of dynamic work in small laboratory animalsu].

A new method of compelling rats and mice to perform uninterrupted dynamic work has been devised. The animal, fixed from its tail and situated in vertical position in respect to the surface of the water with which it comes in contact only with its muzzle and forepaws, is compelled to execute uninterrupted muscular work. Total exhaustion is determined by the cessation of muscular activity, as a result of which the animal, choked by water, perishes.

[Effect of hemorrhagic shock on catecholamine metabolism in the brain].

Content of adrenaline, noradrenaline, dopamine, DOPA and normethanephrine was estimated by means of the hydroxyindole method in brain tissue and hypothalamic region of rats subjected to hemorrhagic shock (loss of 3% of blood during 20 min) within 15 min, 2, 24 and 72 hrs. At the first day after the hemorrhage an increase in content of adrenaline and a distinct decrease in content of noradrenaline were observed in hypothalamic region, although the biosynthesis the neurotransmitters was not inhibited. Then, the noradrenaline concentration was normalized but the content of its precursors was decreased.