Glucuronides Hydrolysis by Intestinal Microbial -Glucuronidases (GUS) Is Affected by Sampling, Enzyme Preparation, Buffer pH, and Species.

Glucuronides hydrolysis by intestinal microbial -Glucuronidases (GUS) is an important procedure for many endogenous and exogenous compounds. The purpose of this study is to determine the impact of experimental conditions on glucuronide hydrolysis by intestinal microbial GUS. Standard probe 4-Nitrophenyl -D-glucopyranoside (pNPG) and a natural glucuronide wogonoside were used as the model compounds.