Publications by authors named "Austin G Seal"
Recombinant adeno-associated viruses (rAAVs) are valuable viral vectors for in vivo gene transfer, also having significant ex vivo therapeutic potential. Continued efforts have focused on various gene therapy applications, capsid engineering, and scalable manufacturing processes. Adherent cells are commonly used for virus production in most basic science laboratories because of their efficiency and cost.
View Article and Find Full Text PDFArticle Synopsis
- Multi-wavelength analytical ultracentrifugation (MW-AUC) offers enhanced accuracy for characterizing and quantifying adeno-associated viruses compared to traditional methods.
- Guidelines for experimental design in sedimentation velocity and buoyant density equilibrium AUC are provided.
- MW-AUC effectively identifies capsid ratios and contaminants, detects partially filled capsids, and delivers more reliable results than dual-wavelength AUC, transmission electron microscopy, and mass photometry, reinforcing its status as the gold standard for analyzing viral vectors.