Gliosis induction on locus coeruleus in a living liver donor experimental model: A brief review.

Living Donor Liver Transplantation (LDLT) is a promising approach to treating end-stage liver diseases, however, some post-operatory complications such as pneumonia, bacteremia, urinary tract infections, and hepatic dysfunction have been reported. In murine models using partial hepatectomy (PHx), a model that emulates LDLT, it has been determined that the synthesis of hepatic cell proliferation factors that are associated with noradrenaline synthesis are produced in locus coeruleus (LC). In addition, studies have shown that PHx decreases GABA and 5-HT2A receptors, promotes loss of dendritic spines, and favors microgliosis in rat hippocampus.

BrdU does not induce hepatocellular damage in experimental Wistar rats.

Bromodeoxyuridine (BrdU) is used in studies related to cell proliferation and neurogenesis. The multiple intraperitoneal injections of this molecule could favor liver function profile changes. In this study, we evaluate the systemic and hepatocellular impact of BrdU in male adult Wistar rats in 30 %-partial hepatectomy (PHx) model.

Caspase-3-related apoptosis prevents pathological regeneration in a living liver donor rat model.

Purpose: The main goal of this study was to determine the relationship of cleaved-caspase-3 (C3)-related apoptosis and hepatic proliferation, during the liver repopulation in a living liver donor rat model.

Material/methods: Thirty-three animals were randomized into eleven groups and evaluated on postoperative from 3 ​h until 384 ​h after 30%-partial hepatectomy (30%-PHx). Liver sections (5 ​μm) were processed by hematoxylin-eosin, and immunostaining for C3, accompanied by hepatic function test.

Optimized technique to extract and fix Olive ridley turtle hatchling retina for histological study.

The efficient extraction and fixation of a tissue allows in preserving the cytoarchitecture, chemical composition and tissue organization, which is key in physiological and histopathological studies. The main goal of this study was to establish a microsurgery technique to obtain ocular tissue and provide an optimized immersion fixation protocol based on the 10 % formalin-intraocular injection on Olive ridley sea turtle hatchlings (Lepidochelys olivacea). To evaluate this optimized technique, a histological comparison between traditional immersion and intraocular/immersion protocols was done.

Chronic consumption of cassava juice induces cellular stress in rat substantia nigra.

Objectives: Cassava ( Crantz) contains cyanogenic glycosides (linamarin and lotaustralin) that have been associated with neurological disorders in humans and rats. In basal ganglia, the dopaminergic neurons of substantia nigra (SNpc) show high cytotoxic susceptibility; therefore, the chronic consumption of cassava (CCC) could induce neurodegeneration in SNpc. In this study we examine the impact of CCC on the integrity of the nigrostriatal system, including apoptosis and microgliosis.